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Top : New Forum Archives (2009-): : Molecular-Cloning
811. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
812. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
813. competent cells stock - (reply: 3)
814. Questions about pIRES-hrGFP1a - (reply: 1)
815. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
816. Cloning into lentiviral vector - (reply: 11)
817. cloning failure help plzzz - cloning (reply: 1)
818. use of pGEM -tT easy vectors - (reply: 2)
819. ~200kb Plasmid DNA purification is not working - (reply: 1)
820. ligating a 6 His tag to plasmid - (reply: 2)
821. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
822. ligation and transformation problem - (reply: 1)
823. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
824. Mini prep of frozen bacteria cells - (reply: 2)
825. PCR cloning problem - could not get colony (reply: 4)
826. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
827. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
828. PCR clonning - (reply: 3)
829. 2 bands in blue colony PCR - (reply: 5)
830. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
831. Introduction to vector development - Basics on vectors types and construction (reply: 1)
832. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
833. Targeted mutagenesis using a linear fragment - (reply: 1)
834. Transformation & Minimal Plates - (reply: 5)
835. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
836. Subcloning Problem - (reply: 3)
837. Restriction Enzyme sites in my genes - (reply: 1)
838. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
839. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
840. In-Fusion (Clontech) Reaction buffer - (reply: 1)