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Top : New Forum Archives (2009-): : Molecular-Cloning
811. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
812. competent cells stock - (reply: 3)
813. Questions about pIRES-hrGFP1a - (reply: 1)
814. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
815. Cloning into lentiviral vector - (reply: 11)
816. cloning failure help plzzz - cloning (reply: 1)
817. use of pGEM -tT easy vectors - (reply: 2)
818. ~200kb Plasmid DNA purification is not working - (reply: 1)
819. ligating a 6 His tag to plasmid - (reply: 2)
820. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
821. ligation and transformation problem - (reply: 1)
822. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
823. Mini prep of frozen bacteria cells - (reply: 2)
824. PCR cloning problem - could not get colony (reply: 4)
825. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
826. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
827. PCR clonning - (reply: 3)
828. 2 bands in blue colony PCR - (reply: 5)
829. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
830. Introduction to vector development - Basics on vectors types and construction (reply: 1)
831. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
832. Targeted mutagenesis using a linear fragment - (reply: 1)
833. Transformation & Minimal Plates - (reply: 5)
834. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
835. Subcloning Problem - (reply: 3)
836. Restriction Enzyme sites in my genes - (reply: 1)
837. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
838. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
839. In-Fusion (Clontech) Reaction buffer - (reply: 1)
840. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)