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Top : New Forum Archives (2009-): : Molecular-Cloning
811. Glycosilated peptide's cDNA cloning - (reply: 3)
812. pWM91 cloning problem - (reply: 4)
813. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
814. Transformation - Transformation (reply: 1)
815. how to add 3XHA to the N-terminal of a protein - (reply: 5)
816. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
817. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
818. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
819. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
820. competent cells stock - (reply: 3)
821. Questions about pIRES-hrGFP1a - (reply: 1)
822. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
823. Cloning into lentiviral vector - (reply: 11)
824. cloning failure help plzzz - cloning (reply: 1)
825. use of pGEM -tT easy vectors - (reply: 2)
826. ~200kb Plasmid DNA purification is not working - (reply: 1)
827. ligating a 6 His tag to plasmid - (reply: 2)
828. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
829. ligation and transformation problem - (reply: 1)
830. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
831. Mini prep of frozen bacteria cells - (reply: 2)
832. PCR cloning problem - could not get colony (reply: 4)
833. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
834. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
835. PCR clonning - (reply: 3)
836. 2 bands in blue colony PCR - (reply: 5)
837. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
838. Introduction to vector development - Basics on vectors types and construction (reply: 1)
839. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
840. Targeted mutagenesis using a linear fragment - (reply: 1)