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Top : New Forum Archives (2009-): : Molecular-Cloning
811. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
812. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
813. PCR clonning - (reply: 3)
814. 2 bands in blue colony PCR - (reply: 5)
815. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
816. Introduction to vector development - Basics on vectors types and construction (reply: 1)
817. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
818. Targeted mutagenesis using a linear fragment - (reply: 1)
819. Transformation & Minimal Plates - (reply: 5)
820. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
821. Subcloning Problem - (reply: 3)
822. Restriction Enzyme sites in my genes - (reply: 1)
823. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
824. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
825. In-Fusion (Clontech) Reaction buffer - (reply: 1)
826. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
827. MDA-MB-231 cell transfection - (reply: 5)
828. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
829. Break Cell Clumps - prepare single cells for injections (reply: 1)
830. 126pb insert clonnig/ digestion issues - (reply: 3)
831. cloning problem... - (reply: 5)
832. yeast glycerol stock - (reply: 2)
833. yeast transformation - (reply: 1)
834. Problems with ligation and transformation - (reply: 2)
835. very small insert and a very ling vector - (reply: 1)
836. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
837. cDNA clone - (reply: 1)
838. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
839. why these guys use T7 instead of CMV? - for transfection (reply: 2)
840. Electroporation - no colonies (reply: 1)