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Top : New Forum Archives (2009-): : Molecular-Cloning
811. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
812. hygromycin concentration for e-coli selection - (reply: 1)
813. orientation of promoter into viral construct - (reply: 1)
814. problems with PCR confirmation of insert . HELP ! - (reply: 1)
815. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
816. Loss of promoter during ligation - (reply: 2)
817. Enzyme restriction - (reply: 8)
818. Colony PCR Problem!!!! - (reply: 5)
819. Removing unwanted sequence from pUC19 - (reply: 4)
820. transformation fails, bacteria grows in control plate - (reply: 6)
821. competentce cell, cloning - (reply: 2)
822. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
823. co-transformation - (reply: 3)
824. pcr clean-up fail? - (reply: 1)
825. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
826. sequential cloning of multiple PCR products - how to do? (reply: 3)
827. Cloning large gene - (reply: 3)
828. Glycosilated peptide's cDNA cloning - (reply: 3)
829. pWM91 cloning problem - (reply: 4)
830. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
831. Transformation - Transformation (reply: 1)
832. how to add 3XHA to the N-terminal of a protein - (reply: 5)
833. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
834. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
835. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
836. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
837. competent cells stock - (reply: 3)
838. Questions about pIRES-hrGFP1a - (reply: 1)
839. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
840. Cloning into lentiviral vector - (reply: 11)