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Top : New Forum Archives (2009-): : Molecular-Cloning
811. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
812. ligation and transformation problem - (reply: 1)
813. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
814. Mini prep of frozen bacteria cells - (reply: 2)
815. PCR cloning problem - could not get colony (reply: 4)
816. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
817. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
818. PCR clonning - (reply: 3)
819. 2 bands in blue colony PCR - (reply: 5)
820. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
821. Introduction to vector development - Basics on vectors types and construction (reply: 1)
822. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
823. Targeted mutagenesis using a linear fragment - (reply: 1)
824. Transformation & Minimal Plates - (reply: 5)
825. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
826. Subcloning Problem - (reply: 3)
827. Restriction Enzyme sites in my genes - (reply: 1)
828. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
829. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
830. In-Fusion (Clontech) Reaction buffer - (reply: 1)
831. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
832. MDA-MB-231 cell transfection - (reply: 5)
833. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
834. Break Cell Clumps - prepare single cells for injections (reply: 1)
835. 126pb insert clonnig/ digestion issues - (reply: 3)
836. cloning problem... - (reply: 5)
837. yeast glycerol stock - (reply: 2)
838. yeast transformation - (reply: 1)
839. Problems with ligation and transformation - (reply: 2)
840. very small insert and a very ling vector - (reply: 1)