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Top : New Forum Archives (2009-): : Molecular-Cloning
811. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
812. Strange results of cloning - insert always reverse-oriented (reply: 2)
813. electroporation - (reply: 2)
814. transfection or infection - difference? (reply: 1)
815. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
816. No colonies after transformation! - (reply: 2)
817. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
818. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
819. Help Miniprep kit SIGMA - (reply: 2)
820. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
821. A question about pBR322 and pUC ori - (reply: 3)
822. Digestion of mini-prep by mistake - (reply: 3)
823. Cloning issue - (reply: 3)
824. Cohesive end filling after CIAP treatment - (reply: 2)
825. Looking for Pfu Taq for high GC-content template - (reply: 7)
826. questions about transformation - (reply: 4)
827. genotype lysogen DE3 - (reply: 1)
828. Need help! - Problem with right insertion! (reply: 1)
829. cloning a large insert - (reply: 5)
830. Transposase contamination in cloning - (reply: 7)
831. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
832. How long can I store cleaved/digested vector? - (reply: 9)
833. Cloning of a gene containing 31 exon - (reply: 1)
834. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
835. Optimum Molar ratio for 3-way ligation - (reply: 5)
836. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
837. cloning help - (reply: 3)
838. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
839. A gene is not inserted into the Plasmid - (reply: 2)
840. Air bubbles during electroporation - (reply: 1)