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Top : New Forum Archives (2009-): : Molecular-Cloning
811. In-Fusion (Clontech) Reaction buffer - (reply: 1)
812. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
813. MDA-MB-231 cell transfection - (reply: 4)
814. Using pcr2.1 Topo (cloning) vector as expression, Huh? - possible? I heard from a PhD student seminar... (reply: 1)
815. Break Cell Clumps - prepare single cells for injections (reply: 1)
816. 126pb insert clonnig/ digestion issues - (reply: 3)
817. cloning problem... - (reply: 5)
818. yeast glycerol stock - (reply: 2)
819. yeast transformation - (reply: 1)
820. Problems with ligation and transformation - (reply: 2)
821. very small insert and a very ling vector - (reply: 1)
822. Guanidine thiocynate contamination in gel extraction - Precipitation vs column (reply: 1)
823. cDNA clone - (reply: 1)
824. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
825. why these guys use T7 instead of CMV? - for transfection (reply: 2)
826. Electroporation - no colonies (reply: 1)
827. enzymes inactivation - (reply: 3)
828. help: why plasmid yield dropped ever since the first culture - (reply: 2)
829. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
830. miRNA cloning - (reply: 2)
831. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
832. Single-digested vector shifts downward on gel - (reply: 1)
833. problem in digestion - (reply: 1)
834. Cloning does not work - (reply: 4)
835. Small Colonies After Transformation - (reply: 5)
836. Ligation and transformation problem - (reply: 2)
837. Agrobacterium EHA101 competent cells - (reply: 1)
838. restriction digestion against colony PCR - (reply: 1)
839. BAC for transgenic mice - (reply: 6)
840. Tag proteins for CO-IP and confocal - (reply: 1)