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Top : New Forum Archives (2009-): : Molecular-Cloning
811. Cloning two inserts at a time in a vector - Cloning two inserts at a time in a vector (reply: 2)
812. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
813. which G418 concentration and treatment period should be used? - (reply: 2)
814. HELP! cloning problem with pET22b using NdeI and XhoI sites - (reply: 4)
815. TOPO TA Cloning of WGA products? - Is this possible? (reply: 2)
816. Chemical competent E.coli always from single colony ? - (reply: 2)
817. midiprep plasmids not expressing - I achieve good yield but now the plasmids do not express (reply: 2)
818. Strange results of cloning - insert always reverse-oriented (reply: 2)
819. electroporation - (reply: 2)
820. transfection or infection - difference? (reply: 1)
821. outgrowth time andampicillin conc for low-copy numbe plasmid - (reply: 15)
822. No colonies after transformation! - (reply: 2)
823. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
824. Proportion of colonies containing DNA inserted at the cloning site - (reply: 1)
825. Help Miniprep kit SIGMA - (reply: 2)
826. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
827. A question about pBR322 and pUC ori - (reply: 3)
828. Digestion of mini-prep by mistake - (reply: 3)
829. Cloning issue - (reply: 3)
830. Cohesive end filling after CIAP treatment - (reply: 2)
831. Looking for Pfu Taq for high GC-content template - (reply: 7)
832. questions about transformation - (reply: 4)
833. genotype lysogen DE3 - (reply: 1)
834. Need help! - Problem with right insertion! (reply: 1)
835. cloning a large insert - (reply: 5)
836. Transposase contamination in cloning - (reply: 7)
837. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
838. How long can I store cleaved/digested vector? - (reply: 9)
839. Cloning of a gene containing 31 exon - (reply: 1)
840. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)