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Top : New Forum Archives (2009-): : Molecular-Cloning
811. cDNA for plasmid transformation PLEASE HElP - (reply: 3)
812. why these guys use T7 instead of CMV? - for transfection (reply: 2)
813. Electroporation - no colonies (reply: 1)
814. enzymes inactivation - (reply: 3)
815. help: why plasmid yield dropped ever since the first culture - (reply: 2)
816. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
817. miRNA cloning - (reply: 2)
818. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
819. Single-digested vector shifts downward on gel - (reply: 1)
820. problem in digestion - (reply: 1)
821. Cloning does not work - (reply: 4)
822. Small Colonies After Transformation - (reply: 5)
823. Ligation and transformation problem - (reply: 2)
824. Agrobacterium EHA101 competent cells - (reply: 1)
825. restriction digestion against colony PCR - (reply: 1)
826. BAC for transgenic mice - (reply: 6)
827. Tag proteins for CO-IP and confocal - (reply: 1)
828. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
829. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
830. how long for a double digestion - (reply: 2)
831. Problems with Site-Directed Mutagenesis - (reply: 2)
832. blunt and sticky end clonning temperature - (reply: 2)
833. Most common mistakes - What mistakes are made during cloning? (reply: 7)
834. Luciferase Assay Stim. Concentration? - (reply: 1)
835. Cloning direction / orientation? - (reply: 2)
836. Question about plasmid midiprep - (reply: 1)
837. Does a polyA sequence need to be put in-frame? - (reply: 1)
838. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
839. Problem on growing up bugs - (reply: 8)
840. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)