Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
811. pcr clean-up fail? - (reply: 1)
812. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
813. sequential cloning of multiple PCR products - how to do? (reply: 3)
814. Cloning large gene - (reply: 3)
815. Glycosilated peptide's cDNA cloning - (reply: 3)
816. pWM91 cloning problem - (reply: 4)
817. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
818. Transformation - Transformation (reply: 1)
819. how to add 3XHA to the N-terminal of a protein - (reply: 5)
820. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
821. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
822. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
823. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
824. competent cells stock - (reply: 3)
825. Questions about pIRES-hrGFP1a - (reply: 1)
826. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
827. Cloning into lentiviral vector - (reply: 11)
828. cloning failure help plzzz - cloning (reply: 1)
829. use of pGEM -tT easy vectors - (reply: 2)
830. ~200kb Plasmid DNA purification is not working - (reply: 1)
831. ligating a 6 His tag to plasmid - (reply: 2)
832. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
833. ligation and transformation problem - (reply: 1)
834. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
835. Mini prep of frozen bacteria cells - (reply: 2)
836. PCR cloning problem - could not get colony (reply: 4)
837. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
838. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
839. PCR clonning - (reply: 3)
840. 2 bands in blue colony PCR - (reply: 5)