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811. ligation and transformation in DH5A - why no colonies after transformation? (reply: 4)
812. overexpression of stable clones - (reply: 1)
813. functionality of yeast promoter in E. coli - (reply: 1)
814. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
815. Can't seem to do a simple cloning - (reply: 2)
816. Histogram from Nanodrop - (reply: 1)
817. cDNA library construction - I get only one colony (reply: 2)
818. Cloning into pIBV5-His (not working) - (reply: 1)
819. Addition of Restriction sites into PCR primers - (reply: 4)
820. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
821. Streaky DNA digest - (reply: 3)
822. TOPO TA cloning problem - (reply: 12)
823. cloning problem - (reply: 2)
824. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
825. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
826. Why did I find a Clonation fragment with a molecular weight unexpected? - (reply: 2)
827. Nco1 digest problem - (reply: 4)
828. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
829. ligation - (reply: 1)
830. pre-prepararation of competent cells - (reply: 3)
831. different copy # multimers with linker ligation? - (reply: 2)
832. Help ADD 3 bases to DNA Sequences - (reply: 6)
833. My battle with BamHI - (reply: 27)
834. serious problem in cloning - (reply: 2)
835. What are "Ella-cre" mice? - (reply: 2)
836. help with pCMV-myc vector - (reply: 2)
837. Elution amount - (reply: 5)
838. keeping restriction digested fragments at -20? - (reply: 5)
839. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)
840. re-digestion - (reply: 1)
812. overexpression of stable clones - (reply: 1)
813. functionality of yeast promoter in E. coli - (reply: 1)
814. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
815. Can't seem to do a simple cloning - (reply: 2)
816. Histogram from Nanodrop - (reply: 1)
817. cDNA library construction - I get only one colony (reply: 2)
818. Cloning into pIBV5-His (not working) - (reply: 1)
819. Addition of Restriction sites into PCR primers - (reply: 4)
820. TA cloning and C-terminal tag - does this work!!! - (reply: 1)
821. Streaky DNA digest - (reply: 3)
822. TOPO TA cloning problem - (reply: 12)
823. cloning problem - (reply: 2)
824. Curious How they do the vector - regarding pTZ57R cloning vector (reply: 2)
825. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
826. Why did I find a Clonation fragment with a molecular weight unexpected? - (reply: 2)
827. Nco1 digest problem - (reply: 4)
828. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
829. ligation - (reply: 1)
830. pre-prepararation of competent cells - (reply: 3)
831. different copy # multimers with linker ligation? - (reply: 2)
832. Help ADD 3 bases to DNA Sequences - (reply: 6)
833. My battle with BamHI - (reply: 27)
834. serious problem in cloning - (reply: 2)
835. What are "Ella-cre" mice? - (reply: 2)
836. help with pCMV-myc vector - (reply: 2)
837. Elution amount - (reply: 5)
838. keeping restriction digested fragments at -20? - (reply: 5)
839. Need help with pkD46, Pkd13, pCP20 - Need Help (reply: 2)
840. re-digestion - (reply: 1)