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Top : New Forum Archives (2009-): : Molecular-Cloning
811. Dissolve IPTG in EtOH - (reply: 4)
812. problem with transient transfection - (reply: 6)
813. Recombinant construct looks smaller than empty vector - (reply: 3)
814. Replacing the CMV promoter of a plasmid with another promoter - (reply: 3)
815. hygromycin concentration for e-coli selection - (reply: 1)
816. orientation of promoter into viral construct - (reply: 1)
817. problems with PCR confirmation of insert . HELP ! - (reply: 1)
818. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
819. Loss of promoter during ligation - (reply: 2)
820. Enzyme restriction - (reply: 8)
821. Colony PCR Problem!!!! - (reply: 5)
822. Removing unwanted sequence from pUC19 - (reply: 4)
823. transformation fails, bacteria grows in control plate - (reply: 6)
824. competentce cell, cloning - (reply: 2)
825. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
826. co-transformation - (reply: 3)
827. pcr clean-up fail? - (reply: 1)
828. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
829. sequential cloning of multiple PCR products - how to do? (reply: 3)
830. Cloning large gene - (reply: 3)
831. Glycosilated peptide's cDNA cloning - (reply: 3)
832. pWM91 cloning problem - (reply: 4)
833. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
834. Transformation - Transformation (reply: 1)
835. how to add 3XHA to the N-terminal of a protein - (reply: 5)
836. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
837. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
838. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
839. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
840. competent cells stock - (reply: 3)