Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
811. problems with PCR confirmation of insert . HELP ! - (reply: 1)
812. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
813. Loss of promoter during ligation - (reply: 2)
814. Enzyme restriction - (reply: 8)
815. Colony PCR Problem!!!! - (reply: 5)
816. Removing unwanted sequence from pUC19 - (reply: 4)
817. transformation fails, bacteria grows in control plate - (reply: 6)
818. competentce cell, cloning - (reply: 2)
819. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
820. co-transformation - (reply: 3)
821. pcr clean-up fail? - (reply: 1)
822. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
823. sequential cloning of multiple PCR products - how to do? (reply: 3)
824. Cloning large gene - (reply: 3)
825. Glycosilated peptide's cDNA cloning - (reply: 3)
826. pWM91 cloning problem - (reply: 4)
827. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
828. Transformation - Transformation (reply: 1)
829. how to add 3XHA to the N-terminal of a protein - (reply: 5)
830. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
831. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
832. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
833. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
834. competent cells stock - (reply: 3)
835. Questions about pIRES-hrGFP1a - (reply: 1)
836. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
837. Cloning into lentiviral vector - (reply: 11)
838. cloning failure help plzzz - cloning (reply: 1)
839. use of pGEM -tT easy vectors - (reply: 2)
840. ~200kb Plasmid DNA purification is not working - (reply: 1)