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Top : New Forum Archives (2009-): : Molecular-Cloning
811. Help Miniprep kit SIGMA - (reply: 2)
812. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
813. A question about pBR322 and pUC ori - (reply: 3)
814. Digestion of mini-prep by mistake - (reply: 3)
815. Cloning issue - (reply: 3)
816. Cohesive end filling after CIAP treatment - (reply: 2)
817. Looking for Pfu Taq for high GC-content template - (reply: 7)
818. questions about transformation - (reply: 4)
819. genotype lysogen DE3 - (reply: 1)
820. Need help! - Problem with right insertion! (reply: 1)
821. cloning a large insert - (reply: 5)
822. Transposase contamination in cloning - (reply: 7)
823. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
824. How long can I store cleaved/digested vector? - (reply: 9)
825. Cloning of a gene containing 31 exon - (reply: 1)
826. A speical ligation case ( a strange ssDNA to dsDNA) - (reply: 4)
827. Optimum Molar ratio for 3-way ligation - (reply: 5)
828. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
829. cloning help - (reply: 3)
830. Blunt end ligation problems - Could the ligation of sticky adaptors help? (reply: 2)
831. A gene is not inserted into the Plasmid - (reply: 2)
832. Air bubbles during electroporation - (reply: 1)
833. uncut plasmid dna vs linearized plasmid gel - (reply: 1)
834. Colonies without inserts - (reply: 9)
835. Transformants Don't Grow - (reply: 4)
836. control DNA transformation- using pGEM kit - transformation (reply: 4)
837. Topo Cloning - Understanding the principle (reply: 6)
838. pMX-PURO Vector not growing - (reply: 3)
839. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)
840. how to set up the ligation - (reply: 14)