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Top : New Forum Archives (2009-): : Molecular-Cloning
811. Transformation of cut plasmid? - need some answers...thank you. (reply: 2)
812. co-transformation - (reply: 3)
813. pcr clean-up fail? - (reply: 1)
814. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
815. sequential cloning of multiple PCR products - how to do? (reply: 3)
816. Cloning large gene - (reply: 3)
817. Glycosilated peptide's cDNA cloning - (reply: 3)
818. pWM91 cloning problem - (reply: 4)
819. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
820. Transformation - Transformation (reply: 1)
821. how to add 3XHA to the N-terminal of a protein - (reply: 5)
822. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
823. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
824. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
825. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
826. competent cells stock - (reply: 3)
827. Questions about pIRES-hrGFP1a - (reply: 1)
828. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
829. Cloning into lentiviral vector - (reply: 11)
830. cloning failure help plzzz - cloning (reply: 1)
831. use of pGEM -tT easy vectors - (reply: 2)
832. ~200kb Plasmid DNA purification is not working - (reply: 1)
833. ligating a 6 His tag to plasmid - (reply: 2)
834. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
835. ligation and transformation problem - (reply: 1)
836. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
837. Mini prep of frozen bacteria cells - (reply: 2)
838. PCR cloning problem - could not get colony (reply: 4)
839. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
840. Sequential digestion with SmaI and EcoRI...... - (reply: 5)