Protocol Online logo
Top : New Forum Archives (2009-): : Molecular-Cloning
811. Cloning large gene - (reply: 3)
812. Glycosilated peptide's cDNA cloning - (reply: 3)
813. pWM91 cloning problem - (reply: 4)
814. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
815. Transformation - Transformation (reply: 1)
816. how to add 3XHA to the N-terminal of a protein - (reply: 5)
817. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
818. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
819. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
820. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
821. competent cells stock - (reply: 3)
822. Questions about pIRES-hrGFP1a - (reply: 1)
823. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
824. Cloning into lentiviral vector - (reply: 11)
825. cloning failure help plzzz - cloning (reply: 1)
826. use of pGEM -tT easy vectors - (reply: 2)
827. ~200kb Plasmid DNA purification is not working - (reply: 1)
828. ligating a 6 His tag to plasmid - (reply: 2)
829. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
830. ligation and transformation problem - (reply: 1)
831. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
832. Mini prep of frozen bacteria cells - (reply: 2)
833. PCR cloning problem - could not get colony (reply: 4)
834. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
835. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
836. PCR clonning - (reply: 3)
837. 2 bands in blue colony PCR - (reply: 5)
838. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
839. Introduction to vector development - Basics on vectors types and construction (reply: 1)
840. Problems with Geneart Site Directed Mutagenesis - (reply: 3)