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Top : New Forum Archives (2009-): : Molecular-Cloning
811. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
812. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
813. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
814. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
815. competent cells stock - (reply: 3)
816. Questions about pIRES-hrGFP1a - (reply: 1)
817. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
818. Cloning into lentiviral vector - (reply: 11)
819. cloning failure help plzzz - cloning (reply: 1)
820. use of pGEM -tT easy vectors - (reply: 2)
821. ~200kb Plasmid DNA purification is not working - (reply: 1)
822. ligating a 6 His tag to plasmid - (reply: 2)
823. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
824. ligation and transformation problem - (reply: 1)
825. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
826. Mini prep of frozen bacteria cells - (reply: 2)
827. PCR cloning problem - could not get colony (reply: 4)
828. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
829. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
830. PCR clonning - (reply: 3)
831. 2 bands in blue colony PCR - (reply: 5)
832. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
833. Introduction to vector development - Basics on vectors types and construction (reply: 1)
834. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
835. Targeted mutagenesis using a linear fragment - (reply: 1)
836. Transformation & Minimal Plates - (reply: 5)
837. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
838. Subcloning Problem - (reply: 3)
839. Restriction Enzyme sites in my genes - (reply: 1)
840. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)