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Top : New Forum Archives (2009-): : Molecular-Cloning
811. electroporation with DH5a vs XL10-Gold - any difference for large plasmids? (reply: 1)
812. sequential cloning of multiple PCR products - how to do? (reply: 3)
813. Cloning large gene - (reply: 3)
814. Glycosilated peptide's cDNA cloning - (reply: 3)
815. pWM91 cloning problem - (reply: 4)
816. Cloudy NZY + Broth maybe contaminated?? - (reply: 4)
817. Transformation - Transformation (reply: 1)
818. how to add 3XHA to the N-terminal of a protein - (reply: 5)
819. Cloning woes...large insert and large vector! - Any help is welcomed (reply: 3)
820. E Coli strain for lentiviral packing vector - >10kb vector not transforming (reply: 4)
821. Is a 3.5 kb fragemnt too big to be cloned ? - (reply: 8)
822. dephosphorylate sticky-ended vector - is it necessary? (reply: 2)
823. competent cells stock - (reply: 3)
824. Questions about pIRES-hrGFP1a - (reply: 1)
825. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
826. Cloning into lentiviral vector - (reply: 11)
827. cloning failure help plzzz - cloning (reply: 1)
828. use of pGEM -tT easy vectors - (reply: 2)
829. ~200kb Plasmid DNA purification is not working - (reply: 1)
830. ligating a 6 His tag to plasmid - (reply: 2)
831. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
832. ligation and transformation problem - (reply: 1)
833. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
834. Mini prep of frozen bacteria cells - (reply: 2)
835. PCR cloning problem - could not get colony (reply: 4)
836. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
837. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
838. PCR clonning - (reply: 3)
839. 2 bands in blue colony PCR - (reply: 5)
840. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)