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Top : New Forum Archives (2009-): : Molecular-Cloning
811. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
812. miRNA cloning - (reply: 2)
813. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
814. Single-digested vector shifts downward on gel - (reply: 1)
815. problem in digestion - (reply: 1)
816. Cloning does not work - (reply: 4)
817. Small Colonies After Transformation - (reply: 5)
818. Ligation and transformation problem - (reply: 2)
819. Agrobacterium EHA101 competent cells - (reply: 1)
820. restriction digestion against colony PCR - (reply: 1)
821. BAC for transgenic mice - (reply: 6)
822. Tag proteins for CO-IP and confocal - (reply: 1)
823. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
824. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
825. how long for a double digestion - (reply: 2)
826. Problems with Site-Directed Mutagenesis - (reply: 2)
827. blunt and sticky end clonning temperature - (reply: 2)
828. Most common mistakes - What mistakes are made during cloning? (reply: 7)
829. Luciferase Assay Stim. Concentration? - (reply: 1)
830. Cloning direction / orientation? - (reply: 2)
831. Question about plasmid midiprep - (reply: 1)
832. Does a polyA sequence need to be put in-frame? - (reply: 1)
833. Is it possible to blunt only either the 5' or 3' end? - (reply: 1)
834. Problem on growing up bugs - (reply: 8)
835. Accidentally using 2 sites w/ compatible ends - Ways to cut down on background? (reply: 2)
836. pSIM in BL21(DE3) - (reply: 2)
837. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
838. Weird problem in invitromutagenesis - (reply: 3)
839. Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit - (reply: 2)
840. RE digestion - (reply: 4)