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Top : New Forum Archives (2009-): : Molecular-Cloning
811. competent cells stock - (reply: 3)
812. Questions about pIRES-hrGFP1a - (reply: 1)
813. transformation and counter selection of yeast mutants - -ura and 5 foa plates (reply: 2)
814. Cloning into lentiviral vector - (reply: 11)
815. cloning failure help plzzz - cloning (reply: 1)
816. use of pGEM -tT easy vectors - (reply: 2)
817. ~200kb Plasmid DNA purification is not working - (reply: 1)
818. ligating a 6 His tag to plasmid - (reply: 2)
819. Plasmid works in miniprep, but not in Maxiprep? - (reply: 3)
820. ligation and transformation problem - (reply: 1)
821. Ligating Incompatible DNA fragments - Ligating Incompatible DNA fragments (reply: 3)
822. Mini prep of frozen bacteria cells - (reply: 2)
823. PCR cloning problem - could not get colony (reply: 4)
824. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
825. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
826. PCR clonning - (reply: 3)
827. 2 bands in blue colony PCR - (reply: 5)
828. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
829. Introduction to vector development - Basics on vectors types and construction (reply: 1)
830. Problems with Geneart Site Directed Mutagenesis - (reply: 3)
831. Targeted mutagenesis using a linear fragment - (reply: 1)
832. Transformation & Minimal Plates - (reply: 5)
833. problems with electroporation of Listeria - please, if you have any ideas.. (reply: 8)
834. Subcloning Problem - (reply: 3)
835. Restriction Enzyme sites in my genes - (reply: 1)
836. TOPO vs. Gateway cloning - Which is the better choice for large inserts. (reply: 4)
837. Mayday Mayday - about the ligation problems - ligation trouble shooting (reply: 3)
838. In-Fusion (Clontech) Reaction buffer - (reply: 1)
839. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
840. MDA-MB-231 cell transfection - (reply: 5)