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Top : New Forum Archives (2009-): : Molecular-Cloning
1621. High conc. CaCl2 inhibits electroporation?? - (reply: 3)
1622. Inserts too small after transformation - transformation (reply: 3)
1623. glycerol stocks - storage (reply: 2)
1624. growth of colonies after ligation - (reply: 2)
1625. cDNA cloning problem - (reply: 1)
1626. Any good way to remove DNase? - (reply: 5)
1627. DH5a comp cells - (reply: 5)
1628. Ligation Problem: Colonies on negative control plate - Lot of colonies on my negative control plate! (reply: 5)
1629. Monocot promoters - (reply: 1)
1630. ligation problem - (reply: 11)
1631. How Gateway cloning system works? - (reply: 1)
1632. Chemically competent to electrocompetent DH5alpha - (reply: 1)
1633. Tetracycline concnetration in selection - (reply: 1)
1634. pCS2 - (reply: 2)
1635. What does this look like to you? - (reply: 23)
1636. ds-oligo cloning/ligation issues - (reply: 18)
1637. Calf Intestinal Alkaline Phosphatase from Fisher - Do I really must inactivate before ligation? (reply: 4)
1638. CIP required for double digestion of plasmid? - (reply: 3)
1639. Help with cloning a plasmid that contains repeats! - (reply: 2)
1640. To what extent competent cells be thawed before transformation? - (reply: 4)
1641. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
1642. size of gene insert wrong? - (reply: 3)
1643. Viral Shuttle Plasmid Restriction digests - (reply: 1)
1644. yeast transformation - how to make a thermotolerant yeast (reply: 4)
1645. need help with klenow - (reply: 4)
1646. Subcloning into pBluescript - (reply: 6)
1647. How to control cloned cDNA expression level in cells? - (reply: 8)
1648. PCR-based Site-directed mutagenesis - clear bend on the gel but no colony on the plate (reply: 7)
1649. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
1650. Help with 3-way ligation - and calculating molar ratios for 3-way ligations... (reply: 11)