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Top : New Forum Archives (2009-): : Molecular-Cloning
1621. pCS2 - (reply: 2)
1622. What does this look like to you? - (reply: 23)
1623. ds-oligo cloning/ligation issues - (reply: 18)
1624. Calf Intestinal Alkaline Phosphatase from Fisher - Do I really must inactivate before ligation? (reply: 4)
1625. CIP required for double digestion of plasmid? - (reply: 3)
1626. Help with cloning a plasmid that contains repeats! - (reply: 2)
1627. To what extent competent cells be thawed before transformation? - (reply: 4)
1628. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
1629. size of gene insert wrong? - (reply: 3)
1630. Viral Shuttle Plasmid Restriction digests - (reply: 1)
1631. yeast transformation - how to make a thermotolerant yeast (reply: 4)
1632. need help with klenow - (reply: 4)
1633. Subcloning into pBluescript - (reply: 6)
1634. How to control cloned cDNA expression level in cells? - (reply: 8)
1635. PCR-based Site-directed mutagenesis - clear bend on the gel but no colony on the plate (reply: 7)
1636. Equation for calculating insert:vector ratio in ligation reaction - (reply: 5)
1637. Help with 3-way ligation - and calculating molar ratios for 3-way ligations... (reply: 11)
1638. Gel purification of vector DNA - low yield - (reply: 26)
1639. ligation and transformation - need help with trouble shooting!!!! (reply: 7)
1640. Identifying cDNA insert - Basic help needed (reply: 2)
1641. gel purification or ligation problem - (reply: 3)
1642. Sequential restriction digestion of plasmid - (reply: 58)
1643. Site-directed mutagenesis - nucleotides insertion (reply: 1)
1644. Troublshooting failed transformation - pLEASE hELP (reply: 19)
1645. Transformation question - (reply: 9)
1646. Cloning trouble - (reply: 5)
1647. Problem cloning oligo into double digested vector - (reply: 9)
1648. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
1649. endonuclease contamination in mini-preps - (reply: 8)
1650. S-O-S Ligation Not Working - No Colonies After Ligation (reply: 5)