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Top : New Forum Archives (2009-): : Molecular-Cloning
1621. No band in the gel after ligation - (reply: 6)
1622. Troubleshooting Digest, ligation, transformation - (reply: 7)
1623. Ideal incubation time - (reply: 3)
1624. Competent DH5 alpha - (reply: 4)
1625. What is the difference between cotransformation and (single transformation to th - What is the difference between cotransformation and single transforma (reply: 2)
1626. Quick ? about Enzyme Buffers - (reply: 3)
1627. High conc. CaCl2 inhibits electroporation?? - (reply: 3)
1628. Inserts too small after transformation - transformation (reply: 3)
1629. glycerol stocks - storage (reply: 2)
1630. growth of colonies after ligation - (reply: 2)
1631. cDNA cloning problem - (reply: 1)
1632. Any good way to remove DNase? - (reply: 5)
1633. DH5a comp cells - (reply: 5)
1634. Ligation Problem: Colonies on negative control plate - Lot of colonies on my negative control plate! (reply: 5)
1635. Monocot promoters - (reply: 1)
1636. ligation problem - (reply: 11)
1637. How Gateway cloning system works? - (reply: 1)
1638. Chemically competent to electrocompetent DH5alpha - (reply: 1)
1639. Tetracycline concnetration in selection - (reply: 1)
1640. pCS2 - (reply: 2)
1641. What does this look like to you? - (reply: 23)
1642. ds-oligo cloning/ligation issues - (reply: 18)
1643. Calf Intestinal Alkaline Phosphatase from Fisher - Do I really must inactivate before ligation? (reply: 4)
1644. CIP required for double digestion of plasmid? - (reply: 3)
1645. Help with cloning a plasmid that contains repeats! - (reply: 2)
1646. To what extent competent cells be thawed before transformation? - (reply: 4)
1647. Problem with the transformation - I could find the insert in plasmid when I did Gel electrophoresis (reply: 1)
1648. size of gene insert wrong? - (reply: 3)
1649. Viral Shuttle Plasmid Restriction digests - (reply: 1)
1650. yeast transformation - how to make a thermotolerant yeast (reply: 4)