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Bacteria Related Discussions
  1. adding Tween80 to bacterial culture for protein purification ?! - (reply: 2)
  2. preparing appropriate dilution of bacteria suspension - (reply: 4)
  3. washing of attached bacterial cells - (reply: 3)
  4. Bacterial Cx grows REALLY Slowly in TB Broth??? - (reply: 1)
  5. Grow multiple Ecoli colonies in a single liquid media tube - (reply: 4)
  6. Growing certain number of bacterial cells in LB - (reply: 1)
  7. Plasmid stability in Ecoli during storage - (reply: 3)
  8. Deactivation of bacteria for competition study - (reply: 2)
  9. 16S ribosomal RNA sequences (Bacteria and Archaea) - (reply: 4)
  10. Test for production of antimicrobial substances by bacteria - (reply: 7)
  11. How to remove attached bacteria (or biofilm) from metallic samples - (reply: 7)
  12. Bacterial Media Formulation - (reply: 2)
  13. Plasmid isolation from gram positive bacteria - (reply: 2)
  14. Agar in plates is contracted after storage and 1 mL of water appears. Any tip? - (reply: 7)
  15. Pelleting bacterial cells - (reply: 1)
  16. Bacterial Motility - (reply: 3)
  17. Any tricks to resuspend bacterial pellets quickly? - (reply: 1)
  18. How to measure immunological markers after bacterial infection; IL10-/- Mice - (reply: 2)
  19. Plasmid isolation from agrobacterium - (reply: 1)
  20. Bacterial growth - (reply: 1)
  21. Can I pause a bacterial culture at 4°C - (reply: 2)
  22. Making a standard curve from bacterial suspensions - (reply: 1)
  23. How to raise polyclonal antibodies against whole bacteria live &/ or inactiv - (reply: 4)
  24. survival strategy of bacteria - (reply: 4)
  25. Break Gram-Negative Bacteria - (reply: 1)
  26. my competent cell grow on any agar plate - (reply: 7)
  27. Bacterial Contamination in HuEmbryonic cell lines seen in SFM4 but not in DMEM + - (reply: 3)
  28. Effect of low and high nutrient medium on bacterial attachment? - (reply: 1)
  29. Storing Bacterial Culture - (reply: 1)
  30. Agrobacterium LBA4404 transformation - (reply: 1)
  31. Calculation Bacteria - (reply: 10)
  32. How to Compare CT values between treated and untreated bacteria with uneven grow - (reply: 1)
  33. How to activate bacteria stored on agar plates? - (reply: 5)
  34. Problems with Ecoli glycerol stock - (reply: 6)
  35. separate plant protoplast from bacteria‏ prior DNA extraction - (reply: 3)
  36. Competent bacterial cells - (reply: 2)
  37. Storing freshly autoclaved LB agar solution? - (reply: 9)
  38. Unknown bacteria??? - (reply: 1)
  39. Diluting Ampicillin into LB Agar - (reply: 24)
  40. Difference between types of bacterial culture broths? - (reply: 4)
  41. SEM analysis of Bacteria - (reply: 3)
  42. How to not get condensation on lid of agar plates? - (reply: 4)
  43. Transformed bacteria grows on plates but not in broth - (reply: 1)
  44. Bacteria specific fluorescent dye - (reply: 5)
  45. Relationship between OD600 and bacterial number - (reply: 1)
  46. Greenish tinge to bacterial culture - (reply: 3)
  47. Fastest way to detect bacteria? - (reply: 15)
  48. If Colony plated on plate coming below agar, is it contamination? - (reply: 13)
  49. Looking for Staib agar dehydrated medium - (reply: 3)
  50. OD range for spreading on agar - (reply: 5)
  51. How to find all Microarray expression probes for Whole genome of Bacteria ( e.g. - (reply: 2)
  52. How to download whole Genome of Bacteria with Individual genes in FASTA format a - (reply: 3)
  53. Bacterial adhesion (attachment) to solid surfaces - (reply: 3)
  54. Textbook Canon: Expressing Any Genes in Bacteria - (reply: 21)
  55. Why does natural bacteria do not give immunity - (reply: 5)
  56. Bacteria grow on MSM agar plate without carbon source - (reply: 1)
  57. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
  58. Preparation of bacterial cells for SEM and TEM - (reply: 2)
  59. Cellophane on agar plate for fungi - (reply: 1)
  60. Quantification of Bacteria Urease Activity??? Berthelot method. - (reply: 1)
  61. Protein Concentration from Bacterial Lysates: Help! - (reply: 2)
  62. Unknown contaminant in Ecoli culture - (reply: 4)
  63. Resuspending bacteria to a certain OD600 - (reply: 10)
  64. May have incorrectly thawed a bacterial culture from -80 C - (reply: 3)
  65. Washing bacteria - (reply: 1)
  66. DNA extration from bacteria using kits or conventional methods - (reply: 6)
  67. Bacillus subtilis growth conditions - (reply: 1)
  68. very low concentraion of DNA from Bacteria - (reply: 3)
  69. Tissue culture contamination like bacteria inside the cells. What is this kind o - (reply: 1)
  70. How to compare the growth rates of different bacteria? - (reply: 3)
  71. Bacterial Operon Knockout - Technique Help - (reply: 2)
  72. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  73. Freezing bacteria - (reply: 4)
  74. White big cloud in agar gel electrophoresis - (reply: 13)
  75. Anaerobic bacteria - (reply: 7)
  76. books for bacteria gene knockout - (reply: 1)
  77. books about bacteria gene knockout - (reply: 9)
  78. Ecoli bacteria storage - (reply: 3)
  79. Growing up bacteria from sewage-lake water in MSM+Antibiotic - (reply: 2)
  80. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
  81. DMSO stock of overnight bacterial cultures - (reply: 1)
  82. inquiry about the methods for gene knockout in bacteria - (reply: 1)
  83. blast bacterial genomes like e.coli - (reply: 1)
  84. how to find suitable vectors to transform wild-type bacteria - (reply: 10)
  85. What OD600 is suitable for assaying Ecoli viability - (reply: 9)
  86. Using pH indicator in agar plates - (reply: 4)
  87. Bacterial Induction - (reply: 1)
  88. How to prepare Gram(+) bacteria for electrotransformation - (reply: 10)
  89. question: RNAi for bacteria? - (reply: 3)
  90. Problem with soft agar assay - (reply: 2)
  91. Quick lysis of gram- bacteria (bugbuster like) - (reply: 3)
  92. reviving bacteria from filter paper - (reply: 1)
  93. bacterial transformation problem - (reply: 2)
  94. YPD + Zeo agar plates - (reply: 2)
  95. bacteria cross-contamination due to aerosol during pipetting - (reply: 3)
  96. Technical question: Why use a bacterial starter culture? - (reply: 5)
  97. isolation of genomic bacterial DNA (bacillus) - (reply: 2)
  98. Agar has dried out during cultivation - (reply: 1)
  99. bacteria - morphology - (reply: 2)
  100. No band after extracting DNA from bacillus subtilis - (reply: 1)
  101. Bacillus subtilis DNA extraction protocol - (reply: 7)
  102. Bacterial expression: incorporating radioactivity into overexpressed protein - (reply: 1)
  103. Glycerol stocks of anaerobic bacteria - (reply: 1)
  104. bacterial transfeomation - (reply: 2)
  105. Soil bacteria - (reply: 3)
  106. Transformation bacteria heat chock and incubation? - (reply: 2)
  107. no bacterial growth after inoculation - (reply: 4)
  108. Blue Bacterial Culture without X-gal - (reply: 3)
  109. How to extract total protein from gram negative bacteria for SDS page and BCA an - (reply: 2)
  110. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
  111. Will bacteria survive if unmixed with glycerol in -80 Celsius - (reply: 1)
  112. Bacteria storage - (reply: 10)
  113. how to determine a plant promoter activity in bacteria - (reply: 12)
  114. transformation fails, bacteria grows in control plate - (reply: 6)
  115. Thermophilic Bacteria - (reply: 3)
  116. Question about measuring bacterial OD - requensing inputs on measurement volume (reply: 10)
  117. measuring the serum Ig concentration and bacteria infection - (reply: 1)
  118. Scrape Bacteria from Agar plate - (reply: 2)
  119. Mini prep of frozen bacteria cells - (reply: 2)
  120. Electroporation for Marine bacteria, Help me! - (reply: 6)
  121. Bacterial preservation - Bacterial preservation (reply: 1)
  122. bacterial Cell free culture supernatants - Can any one help me out to prepare a cell free culture supernatant (reply: 3)
  123. Would clean dry hot air kill bacteria? - (reply: 6)
  124. Microscope for bacteria and yeast - (reply: 1)
  125. Determination of bacteria concentration for inducing Immune response - The relevance of LD-50 in inducing immune response (reply: 14)
  126. no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)
  127. Agrobacterium EHA101 competent cells - (reply: 1)
  128. centrifuge, bacterial cells - what is the highest centrifugal force (rcf) (reply: 4)
  129. bacterial growth in buffers - (reply: 2)
  130. positive or negative mannitol salt agar plate??!! - (reply: 3)
  131. Isolating thermophilic bacteria - (reply: 2)
  132. Problem with construction of deletion mutant (Bacteria) - Deletion mutant, pK19mobsacB, Corynebacterium glutamicum (reply: 4)
  133. Use of bacteria in percious metal reclamination - (reply: 4)
  134. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
  135. Bacterial DNA Extraction - possible of contamination? - (reply: 7)
  136. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
  137. 23S rRNA for bacteria - (reply: 1)
  138. Why shouldn't you clone using expression bacteria - (reply: 6)
  139. Dissolving Cholesterol in MSM Agar - (reply: 2)
  140. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
  141. CALCULATING no of bacteria in original sample...after dilutions? [Very SHORT Que - Simple to you but please give a hint! (reply: 6)
  142. Lipid extraction from bacteria - Lipid extraction from bacteria (reply: 1)
  143. Lipid secreting bacteria - Lipid secreting bacteria (reply: 1)
  144. worried about the p. acne bacteria, please help - (reply: 3)
  145. Get rid of yeast but not the bacteria - how? - (reply: 4)
  146. mold contamination in my bacterial culture - (reply: 16)
  147. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)
  148. Semi solid agar selection of stable transfectant - antibiotic?? - (reply: 3)
  149. subcloning into bacterial expression vector - (reply: 1)
  150. Bacterial lysis buffer - (reply: 3)
  151. Problems with Bacterial Induction: NO Induction - (reply: 1)
  152. Bacteria to Cell Culture - How many bacteria should I add to cell culture (reply: 2)
  153. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
  154. Catechol 1,2 Dioxygenase Activity in Bacterial Isolate - (reply: 3)
  155. Bacterial Induction: Same concentration of protein samples? - (reply: 1)
  156. Rabbit cells to grow bacteria in before immunization of rabbit for antibody prod - (reply: 2)
  157. construction of a bacterial isogenic mutant - construction of a bacterial isogenic mutant (reply: 2)
  158. Repeat bacterial infections - Trying to find the source (reply: 7)
  159. Biochemical tests for unknown bacteria - (reply: 1)
  160. How to prepare SOB agar plate - If it possible to add all chemicals before autoclaving (reply: 1)
  161. Isolating bacterial RNA and protein from the host tissue sample - (reply: 3)
  162. Do plasmids work in bacteria? - (reply: 1)
  163. Viability of intracellular bacteria - (reply: 1)
  164. recombinant ecoli in log phase overnight media suggestion - (reply: 2)
  165. Remove iron from agar? - (reply: 2)
  166. DNA Extraction from bacterial cultures - (reply: 3)
  167. Is it possible to know the live bacteria using PCR for a certain genus? - (reply: 5)
  168. Is it possible to know only the live bacteria using PCR for a certain genus? - (reply: 1)
  169. Extraction of bacterial DNA - (reply: 1)
  170. How to analyze a food sample for possible bacterial contamination - Food that is involved in a minor outbreak (reply: 4)
  171. Storage of Bacterial Culture at 4 degrees - (reply: 2)
  172. transcription and translation questions - when do they start and stop in bacteria? (reply: 1)
  173. Cleaning contaminated DNA - separating human from bacterial DNA (reply: 1)
  174. Differentiating Ecoli from Agrobacteria quickly. - Noob mistake, I am paying for now. (reply: 2)
  175. bacterial identification using real time PCR - (reply: 1)
  176. Eliminate bacteria(l DNA) from hypochlorite-treated nematode eggs - (reply: 2)
  177. grow in broth but not on agar... - after transformation (reply: 2)
  178. bacteria inhibiting Staphyllococcus aureus - what to do more (reply: 6)
  179. bacterial culture - (reply: 4)
  180. soft agar assay - cells in the top agar cells adhere to the plates unexpectedly. (reply: 4)
  181. transformed bacteria doesnt grow - (reply: 1)
  182. Storage of bacteria in fetal calf serum - 70 or - 80 ? - Temperature (reply: 2)
  183. Long term storage of bacteria in fetal calf serum -70 or -80 - Temperature issues (reply: 3)
  184. How to extract restriction enzyme (or restriction endonuclease) from bacteria? - (reply: 1)
  185. Blast Nucleotide Issue - No more Gene ID when comparing Bacteria genome (reply: 3)
  186. Quantifying bacterial infection microscopically - (reply: 2)
  187. Bacteria Media (NZY+ broth) - (reply: 1)
  188. Inquiry about Obtaining reference bacterial strains - (reply: 1)
  189. Bacteria growing on plate but not liquid media - (reply: 8)
  190. Bacteria and Amaxa - (reply: 2)
  191. Transporting some bacteria cells - transport bacteria cells pellet (reply: 2)
  192. Venter did it again - creating a bacterium... (reply: 2)
  193. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  194. Southern blot help--no signal - for bacterial KO verification (reply: 2)
  195. fixating bacterial spores - (reply: 4)
  196. best way to transport bacteria at room temperature - (reply: 5)
  197. Making glycerol stock of bacteria - storage issue - (reply: 8)
  198. Extremely small bacterial colonies after transformation - (reply: 10)
  199. questions about soft agar assay - (reply: 1)
  200. Agar problems..... extremely desperate. - Low-phosphate agar not setting (reply: 3)
  201. Bacterial invasion assay with human cells - (reply: 2)
  202. Bacteria culture and growth curve? - (reply: 1)
  203. Bacteria culture-Glass or Plastic Erlenmeyer flasks - What do you prefer Glass or Plastic Erlenmeyer flasks? (reply: 7)
  204. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
  205. ampicillin for LB agar - (reply: 2)
  206. spread bacteria on agar plate until it is dry? - or if it is still wet is ok? (a very basic question) (reply: 3)
  207. How to get consistent bacterial growth - (reply: 1)
  208. Colonies on Agar plate. - (reply: 3)
  209. DNA extration from Bacteria spores - (reply: 13)
  210. Keeping agar in liquid form - problems? - (reply: 5)
  211. Purification of scretory protein from bacteria - (reply: 2)
  212. How long does Erythromycin last in an agar plate - (reply: 2)
  213. Please help--possible bacteria issue--cholesterol + red blood cells involved - (reply: 1)
  214. Bacterial growth in agar plate with ampicillin - (reply: 4)
  215. Help for an Organic Chemist - Question about bacteria cell wall permeability. (reply: 6)
  216. Bacteria transformation - (reply: 1)
  217. EDTA bacterial cell lysate - (reply: 2)
  218. Sodium Hypochlorite to kill bacteria - Alternatives to autoclaving bacterial cultures (reply: 6)
  219. how long is carb stable in liquid broth at 37C - I need longer incubation times of bacteria because of the transformant (reply: 1)
  220. CLED Agar Help Please - (reply: 1)
  221. Lysis of infected macrophages - without lysing bacteria (reply: 2)
  222. Growing bacterial culture - problem encountered in growing staphylococcus epidermidis (reply: 2)
  223. Bacteria Lysis Reagent For RNA extraction - Need Expert's Opinion (reply: 12)
  224. Isolation of bacteria from breast milk - (reply: 2)
  225. deleting a certain gene from bacterium - (reply: 12)
  226. What fields of research other than microbiology work with bacteria and - (reply: 1)
  227. How to inoculate bacteria? - (reply: 10)
  228. ideas for Fluorescent tagging of 7Kda bacterial protein. - (reply: 1)
  229. Food colouring agar plates - Do you think it will affect cells? (reply: 11)
  230. Storing blood to be used for culturing bacteria - (reply: 3)
  231. what bacteria? - (reply: 5)
  232. bacteria lysis - E coli lysis for recombinant protein extraction (reply: 1)
  233. SOS....Genomic DNA extraction - Extraction from gram positive bacillus stearothermophilus (reply: 8)
  234. bacterial infectious diseases - (reply: 3)
  235. LB-Cm Agar plates - I know what LB is, what is Cm? (reply: 5)
  236. bacterial stock preparation - (reply: 3)
  237. Help! Bacteria on agar not growing in LB broth - even without antibiotics... (reply: 3)
  238. Moisture in Agar plates! - (reply: 3)
  239. look for E.coli DB3.1 bacteria - (reply: 2)
  240. Bacterial promoter prediction - (reply: 2)
  241. how to isolate insoluble protein in bacterial induction? - (reply: 4)
  242. Bacteria from hot water spring - what to do (reply: 2)
  243. Longevity of plain LB agar plates - (reply: 10)
  244. Endogenous control in bacterial RT-qPCR - (reply: 4)
  245. Some of the agar plates were not solid! >:( - Another bad day for science!! (reply: 10)
  246. Need Help: Bacterial gene deletion- sacB sucrose resistance - (reply: 31)
  247. methylotrophic bacteria culture contamination - (reply: 1)
  248. Suitable bacterial reporter vectors - (reply: 3)
  249. where can bacillus coagulans live?its habitant - Hi...all Can you please provide me sources of bacillus coagulans for (reply: 1)
  250. bacterial viability assay - (reply: 4)
  251. radio-active substances , bacteria - (reply: 9)
  252. Bacteria culture - OD, time (reply: 5)
  253. Strange result on Agar Plate - (reply: 5)
  254. Fixation of GFP bacteria - (reply: 2)
  255. Bacteria Changing Colour - (reply: 7)
  256. Bacillus coagulans identification - (reply: 1)
  257. Culture of soil bacteria - (reply: 11)
  258. Quantifying ingested bacteria without realtime PCR - (reply: 3)
  259. bacterial contamination in cell culture - (reply: 3)
  260. Harvest intracellular bacterial RNA - (reply: 2)
  261. frozen bacteria in glycerol - quickly shock in liquid nitrogen? (reply: 7)
  262. agar polymerization - (reply: 4)
  263. Identification if these bacteria are sporeforming or nonsporeforming. - (reply: 6)
  264. OD of bacteria at different culture volume - (reply: 6)
  265. Electrocompetent bacteria - (reply: 1)
  266. PCR screening of transformed bacterial colony - (reply: 6)
  267. Use of Ampicillin in agar for E.coli - E.coli did grow in +ve and -ve control (reply: 8)
  268. Bacterial strain stocks - (reply: 2)
  269. Isolating bacteria from grass roots - (reply: 5)
  270. YOU SHALL NOT PASS!! - bacteria will not transform..I think:/ (reply: 4)
  271. Quantifying filamentous bacteria growth - (reply: 6)
  272. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  273. Resuspend bacterial pellet - (reply: 4)
  274. Thermal stability of H202 - stability of H2O2 in hot agar (reply: 1)
  275. soil sampling for bacterial diversity study - (reply: 4)
  276. Yellow E. coli colonies on MACCONKEY AGAR - (reply: 2)
  277. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
  278. Bacteria pellet has almost-black region - (reply: 10)
  279. storing agar plates - why upside down? (reply: 5)
  280. Bacteria gene expression - (reply: 2)
  281. Bacterial Staining and Counting - (reply: 1)
  282. COLIFORM ISSUES - coliforms, bacteria (reply: 1)
  283. 1.5% agar plate - (reply: 10)
  284. counting bacterial colonies - (reply: 5)
  285. dmso in bacterial stock - (reply: 1)
  286. Frozen stock of bacteria - Can all bacteria be prepared as frozen stock ? (reply: 10)
  287. Live/dead bacteria count - without using fluorescence (reply: 1)
  288. Frozen bacterial stock - (reply: 3)
  289. Identification of a bacteria - How to determine wich bacteria you are working with (reply: 3)
  290. bacterial strains - L.lactis MG1363(^Htra mutant) or L.lactis NZ9000(^Htra mutant) (reply: 1)
  291. fungus or bacteria Database - wich one do you use? (reply: 7)
  292. Bubbles in my agar plates after drying - Or "how I knew I have a poltergeist at lab"... (reply: 7)
  293. Isolating bacteria from roots - (reply: 1)
  294. Commercially available bacterial expression system with chaperone coexpression - (reply: 1)
  295. contamination in transformed bacteria - black filamentous fungi grows when culturing competent bacteria with m (reply: 8)
  296. Volume of antibiotics per agar plate - (reply: 9)
  297. bacterial growth - (reply: 1)
  298. Tips for genomic DNA extraction from gram -ve bacteria - (reply: 2)
  299. Bacterial conjugation - between E. coli and Salmonella Typhimurium (reply: 1)
  300. Solvent for Agar - is there a solvent for dissolving agar (reply: 6)
  301. condensation on agar plates - what happens if it drops in the plate while plating (reply: 6)
  302. Effect of salt concentration on bacterial culture - (reply: 4)
  303. Measuring OD for a bacteria culture - Directly or diluted? (reply: 7)
  304. Chilling Bacterial Cultures Before Centrifugation - (reply: 3)
  305. Stored bacteria lost plasmids? - (reply: 12)
  306. Centrifuging to separate bacteria from molecules - (reply: 14)
  307. RNAi in bacteria - Why no finding for gene silencing in bacteria? (reply: 1)