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Bacteria Related Discussions
  1. Bacterial Operon Knockout - Technique Help - (reply: 1)
  2. Plasmid with two similar bacterial ORIs - Can it be transformed into bacteria?? - (reply: 2)
  3. Freezing bacteria - (reply: 4)
  4. White big cloud in agar gel electrophoresis - (reply: 13)
  5. Anaerobic bacteria - (reply: 7)
  6. books about bacteria gene knockout - (reply: 9)
  7. Ecoli bacteria storage - (reply: 3)
  8. Growing up bacteria from sewage-lake water in MSM+Antibiotic - (reply: 2)
  9. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
  10. DMSO stock of overnight bacterial cultures - (reply: 1)
  11. blast bacterial genomes like e.coli - (reply: 1)
  12. how to find suitable vectors to transform wild-type bacteria - (reply: 10)
  13. What OD600 is suitable for assaying Ecoli viability - (reply: 9)
  14. Using pH indicator in agar plates - (reply: 4)
  15. Bacterial Induction - (reply: 1)
  16. How to prepare Gram(+) bacteria for electrotransformation - (reply: 10)
  17. question: RNAi for bacteria? - (reply: 3)
  18. Problem with soft agar assay - (reply: 2)
  19. Quick lysis of gram- bacteria (bugbuster like) - (reply: 3)
  20. reviving bacteria from filter paper - (reply: 1)
  21. bacterial transformation problem - (reply: 2)
  22. YPD + Zeo agar plates - (reply: 2)
  23. bacteria cross-contamination due to aerosol during pipetting - (reply: 3)
  24. Technical question: Why use a bacterial starter culture? - (reply: 5)
  25. isolation of genomic bacterial DNA (bacillus) - (reply: 2)
  26. Agar has dried out during cultivation - (reply: 1)
  27. bacteria - morphology - (reply: 2)
  28. No band after extracting DNA from bacillus subtilis - (reply: 1)
  29. Bacillus subtilis DNA extraction protocol - (reply: 7)
  30. Bacterial expression: incorporating radioactivity into overexpressed protein - (reply: 1)
  31. Glycerol stocks of anaerobic bacteria - (reply: 1)
  32. bacterial transfeomation - (reply: 2)
  33. Soil bacteria - (reply: 3)
  34. Transformation bacteria heat chock and incubation? - (reply: 2)
  35. no bacterial growth after inoculation - (reply: 4)
  36. Blue Bacterial Culture without X-gal - (reply: 3)
  37. How to extract total protein from gram negative bacteria for SDS page and BCA an - (reply: 2)
  38. Transforming bacteria with plasmid from filter paper, months later - (reply: 5)
  39. Will bacteria survive if unmixed with glycerol in -80 Celsius - (reply: 1)
  40. Bacteria storage - (reply: 10)
  41. how to determine a plant promoter activity in bacteria - (reply: 12)
  42. transformation fails, bacteria grows in control plate - (reply: 6)
  43. Thermophilic Bacteria - (reply: 3)
  44. Question about measuring bacterial OD - requensing inputs on measurement volume (reply: 8)
  45. measuring the serum Ig concentration and bacteria infection - (reply: 2)
  46. Scrape Bacteria from Agar plate - (reply: 2)
  47. Mini prep of frozen bacteria cells - (reply: 2)
  48. Electroporation for Marine bacteria, Help me! - (reply: 6)
  49. Bacterial preservation - Bacterial preservation (reply: 1)
  50. bacterial Cell free culture supernatants - Can any one help me out to prepare a cell free culture supernatant (reply: 3)
  51. Would clean dry hot air kill bacteria? - (reply: 6)
  52. Microscope for bacteria and yeast - (reply: 1)
  53. Determination of bacteria concentration for inducing Immune response - The relevance of LD-50 in inducing immune response (reply: 14)
  54. no band observed for bacterial genomic DNA during agarose gel electrophoresis - (reply: 9)
  55. Agrobacterium EHA101 competent cells - (reply: 1)
  56. centrifuge, bacterial cells - what is the highest centrifugal force (rcf) (reply: 4)
  57. bacterial growth in buffers - (reply: 2)
  58. positive or negative mannitol salt agar plate??!! - (reply: 3)
  59. Isolating thermophilic bacteria - (reply: 2)
  60. Problem with construction of deletion mutant (Bacteria) - Deletion mutant, pK19mobsacB, Corynebacterium glutamicum (reply: 4)
  61. Use of bacteria in percious metal reclamination - (reply: 4)
  62. pLys plasmid containing bacterial strains - Can I use these strains for plasmid preps? (reply: 1)
  63. Bacterial DNA Extraction - possible of contamination? - (reply: 7)
  64. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
  65. 23S rRNA for bacteria - (reply: 1)
  66. Why shouldn't you clone using expression bacteria - (reply: 6)
  67. Dissolving Cholesterol in MSM Agar - (reply: 2)
  68. Resistant strains can grow on Ampicillin agar plate but cannot grow in LB medium - (reply: 6)
  69. CALCULATING no of bacteria in original sample...after dilutions? [Very SHORT Que - Simple to you but please give a hint! (reply: 6)
  70. Lipid extraction from bacteria - Lipid extraction from bacteria (reply: 1)
  71. Lipid secreting bacteria - Lipid secreting bacteria (reply: 1)
  72. worried about the p. acne bacteria, please help - (reply: 3)
  73. Get rid of yeast but not the bacteria - how? - (reply: 4)
  74. mold contamination in my bacterial culture - (reply: 16)
  75. Centrifuging bacterial cultures - Fill those bottles! (reply: 2)
  76. Semi solid agar selection of stable transfectant - antibiotic?? - (reply: 3)
  77. subcloning into bacterial expression vector - (reply: 1)
  78. Bacterial lysis buffer - (reply: 3)
  79. Problems with Bacterial Induction: NO Induction - (reply: 1)
  80. Bacteria to Cell Culture - How many bacteria should I add to cell culture (reply: 2)
  81. why must we use freshly streak but not old/spread bacteria? - (reply: 6)
  82. Catechol 1,2 Dioxygenase Activity in Bacterial Isolate - (reply: 3)
  83. Bacterial Induction: Same concentration of protein samples? - (reply: 1)
  84. Rabbit cells to grow bacteria in before immunization of rabbit for antibody prod - (reply: 2)
  85. construction of a bacterial isogenic mutant - construction of a bacterial isogenic mutant (reply: 2)
  86. Repeat bacterial infections - Trying to find the source (reply: 7)
  87. Biochemical tests for unknown bacteria - (reply: 1)
  88. How to prepare SOB agar plate - If it possible to add all chemicals before autoclaving (reply: 1)
  89. Isolating bacterial RNA and protein from the host tissue sample - (reply: 3)
  90. Do plasmids work in bacteria? - (reply: 1)
  91. Viability of intracellular bacteria - (reply: 1)
  92. recombinant ecoli in log phase overnight media suggestion - (reply: 2)
  93. Remove iron from agar? - (reply: 2)
  94. DNA Extraction from bacterial cultures - (reply: 3)
  95. Is it possible to know the live bacteria using PCR for a certain genus? - (reply: 5)
  96. Is it possible to know only the live bacteria using PCR for a certain genus? - (reply: 1)
  97. Extraction of bacterial DNA - (reply: 1)
  98. How to analyze a food sample for possible bacterial contamination - Food that is involved in a minor outbreak (reply: 4)
  99. Storage of Bacterial Culture at 4 degrees - (reply: 2)
  100. transcription and translation questions - when do they start and stop in bacteria? (reply: 1)
  101. Cleaning contaminated DNA - separating human from bacterial DNA (reply: 1)
  102. Differentiating Ecoli from Agrobacteria quickly. - Noob mistake, I am paying for now. (reply: 2)
  103. bacterial identification using real time PCR - (reply: 1)
  104. Eliminate bacteria(l DNA) from hypochlorite-treated nematode eggs - (reply: 2)
  105. grow in broth but not on agar... - after transformation (reply: 2)
  106. bacteria inhibiting Staphyllococcus aureus - what to do more (reply: 6)
  107. bacterial culture - (reply: 4)
  108. soft agar assay - cells in the top agar cells adhere to the plates unexpectedly. (reply: 4)
  109. transformed bacteria doesnt grow - (reply: 1)
  110. Storage of bacteria in fetal calf serum - 70 or - 80 ? - Temperature (reply: 2)
  111. Long term storage of bacteria in fetal calf serum -70 or -80 - Temperature issues (reply: 3)
  112. How to extract restriction enzyme (or restriction endonuclease) from bacteria? - (reply: 1)
  113. Blast Nucleotide Issue - No more Gene ID when comparing Bacteria genome (reply: 3)
  114. Quantifying bacterial infection microscopically - (reply: 2)
  115. Bacteria Media (NZY+ broth) - (reply: 1)
  116. Inquiry about Obtaining reference bacterial strains - (reply: 1)
  117. Bacteria growing on plate but not liquid media - (reply: 8)
  118. Bacteria and Amaxa - (reply: 2)
  119. Transporting some bacteria cells - transport bacteria cells pellet (reply: 2)
  120. Venter did it again - creating a bacterium... (reply: 2)
  121. problems in cloning, need help - problems with plasmid stability in bacteria (reply: 4)
  122. Southern blot help--no signal - for bacterial KO verification (reply: 2)
  123. fixating bacterial spores - (reply: 4)
  124. best way to transport bacteria at room temperature - (reply: 5)
  125. Making glycerol stock of bacteria - storage issue - (reply: 8)
  126. Extremely small bacterial colonies after transformation - (reply: 10)
  127. questions about soft agar assay - (reply: 1)
  128. Agar problems..... extremely desperate. - Low-phosphate agar not setting (reply: 3)
  129. Bacterial invasion assay with human cells - (reply: 2)
  130. Bacteria culture and growth curve? - (reply: 1)
  131. Bacteria culture-Glass or Plastic Erlenmeyer flasks - What do you prefer Glass or Plastic Erlenmeyer flasks? (reply: 7)
  132. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
  133. ampicillin for LB agar - (reply: 2)
  134. spread bacteria on agar plate until it is dry? - or if it is still wet is ok? (a very basic question) (reply: 3)
  135. How to get consistent bacterial growth - (reply: 1)
  136. Colonies on Agar plate. - (reply: 3)
  137. DNA extration from Bacteria spores - (reply: 13)
  138. Keeping agar in liquid form - problems? - (reply: 5)
  139. Purification of scretory protein from bacteria - (reply: 2)
  140. How long does Erythromycin last in an agar plate - (reply: 2)
  141. Please help--possible bacteria issue--cholesterol + red blood cells involved - (reply: 1)
  142. Bacterial growth in agar plate with ampicillin - (reply: 4)
  143. Help for an Organic Chemist - Question about bacteria cell wall permeability. (reply: 6)
  144. Bacteria transformation - (reply: 1)
  145. EDTA bacterial cell lysate - (reply: 2)
  146. Sodium Hypochlorite to kill bacteria - Alternatives to autoclaving bacterial cultures (reply: 6)
  147. how long is carb stable in liquid broth at 37C - I need longer incubation times of bacteria because of the transformant (reply: 1)
  148. CLED Agar Help Please - (reply: 1)
  149. Lysis of infected macrophages - without lysing bacteria (reply: 2)
  150. Growing bacterial culture - problem encountered in growing staphylococcus epidermidis (reply: 2)
  151. Bacteria Lysis Reagent For RNA extraction - Need Expert's Opinion (reply: 12)
  152. Isolation of bacteria from breast milk - (reply: 2)
  153. deleting a certain gene from bacterium - (reply: 12)
  154. What fields of research other than microbiology work with bacteria and - (reply: 1)
  155. How to inoculate bacteria? - (reply: 10)
  156. ideas for Fluorescent tagging of 7Kda bacterial protein. - (reply: 1)
  157. Food colouring agar plates - Do you think it will affect cells? (reply: 11)
  158. Storing blood to be used for culturing bacteria - (reply: 3)
  159. what bacteria? - (reply: 5)
  160. bacteria lysis - E coli lysis for recombinant protein extraction (reply: 1)
  161. SOS....Genomic DNA extraction - Extraction from gram positive bacillus stearothermophilus (reply: 8)
  162. bacterial infectious diseases - (reply: 3)
  163. LB-Cm Agar plates - I know what LB is, what is Cm? (reply: 5)
  164. bacterial stock preparation - (reply: 3)
  165. Help! Bacteria on agar not growing in LB broth - even without antibiotics... (reply: 3)
  166. Moisture in Agar plates! - (reply: 3)
  167. look for E.coli DB3.1 bacteria - (reply: 2)
  168. Bacterial promoter prediction - (reply: 2)
  169. fixing bacterial cells in ELISA - (reply: 1)
  170. how to isolate insoluble protein in bacterial induction? - (reply: 4)
  171. Bacteria from hot water spring - what to do (reply: 2)
  172. Longevity of plain LB agar plates - (reply: 10)
  173. Endogenous control in bacterial RT-qPCR - (reply: 4)
  174. Some of the agar plates were not solid! >:( - Another bad day for science!! (reply: 10)
  175. Need Help: Bacterial gene deletion- sacB sucrose resistance - (reply: 31)
  176. methylotrophic bacteria culture contamination - (reply: 1)
  177. Suitable bacterial reporter vectors - (reply: 3)
  178. where can bacillus coagulans live?its habitant - Hi...all Can you please provide me sources of bacillus coagulans for (reply: 1)
  179. bacterial viability assay - (reply: 4)
  180. radio-active substances , bacteria - (reply: 9)
  181. Bacteria culture - OD, time (reply: 5)
  182. Strange result on Agar Plate - (reply: 5)
  183. Fixation of GFP bacteria - (reply: 2)
  184. Bacteria Changing Colour - (reply: 7)
  185. Bacillus coagulans identification - (reply: 1)
  186. Culture of soil bacteria - (reply: 11)
  187. Quantifying ingested bacteria without realtime PCR - (reply: 3)
  188. bacterial contamination in cell culture - (reply: 3)
  189. Harvest intracellular bacterial RNA - (reply: 2)
  190. frozen bacteria in glycerol - quickly shock in liquid nitrogen? (reply: 7)
  191. agar polymerization - (reply: 4)
  192. Identification if these bacteria are sporeforming or nonsporeforming. - (reply: 6)
  193. OD of bacteria at different culture volume - (reply: 6)
  194. Electrocompetent bacteria - (reply: 1)
  195. PCR screening of transformed bacterial colony - (reply: 6)
  196. Use of Ampicillin in agar for E.coli - E.coli did grow in +ve and -ve control (reply: 8)
  197. Bacterial strain stocks - (reply: 2)
  198. Isolating bacteria from grass roots - (reply: 5)
  199. YOU SHALL NOT PASS!! - bacteria will not transform..I think:/ (reply: 4)
  200. Quantifying filamentous bacteria growth - (reply: 6)
  201. bacterial is white, and no plasmid in it - problem with ligation (reply: 2)
  202. Resuspend bacterial pellet - (reply: 4)
  203. Thermal stability of H202 - stability of H2O2 in hot agar (reply: 1)
  204. soil sampling for bacterial diversity study - (reply: 4)
  205. Yellow E. coli colonies on MACCONKEY AGAR - (reply: 2)
  206. What does the DH5alpha mean in E.coli DH5alpha bacteria - (reply: 10)
  207. Bacteria pellet has almost-black region - (reply: 10)
  208. storing agar plates - why upside down? (reply: 5)
  209. Bacteria gene expression - (reply: 2)
  210. Bacterial Staining and Counting - (reply: 1)
  211. COLIFORM ISSUES - coliforms, bacteria (reply: 1)
  212. 1.5% agar plate - (reply: 10)
  213. counting bacterial colonies - (reply: 5)
  214. dmso in bacterial stock - (reply: 1)
  215. Frozen stock of bacteria - Can all bacteria be prepared as frozen stock ? (reply: 10)
  216. Live/dead bacteria count - without using fluorescence (reply: 1)
  217. Frozen bacterial stock - (reply: 3)
  218. Identification of a bacteria - How to determine wich bacteria you are working with (reply: 3)
  219. bacterial strains - L.lactis MG1363(^Htra mutant) or L.lactis NZ9000(^Htra mutant) (reply: 1)
  220. fungus or bacteria Database - wich one do you use? (reply: 7)
  221. Bubbles in my agar plates after drying - Or "how I knew I have a poltergeist at lab"... (reply: 7)
  222. Isolating bacteria from roots - (reply: 1)
  223. Commercially available bacterial expression system with chaperone coexpression - (reply: 1)
  224. contamination in transformed bacteria - black filamentous fungi grows when culturing competent bacteria with m (reply: 8)
  225. Volume of antibiotics per agar plate - (reply: 9)
  226. bacterial growth - (reply: 1)
  227. Tips for genomic DNA extraction from gram -ve bacteria - (reply: 2)
  228. Bacterial conjugation - between E. coli and Salmonella Typhimurium (reply: 1)
  229. Solvent for Agar - is there a solvent for dissolving agar (reply: 6)
  230. condensation on agar plates - what happens if it drops in the plate while plating (reply: 6)
  231. Effect of salt concentration on bacterial culture - (reply: 4)
  232. Measuring OD for a bacteria culture - Directly or diluted? (reply: 7)
  233. Chilling Bacterial Cultures Before Centrifugation - (reply: 3)
  234. Stored bacteria lost plasmids? - (reply: 12)
  235. Centrifuging to separate bacteria from molecules - (reply: 14)
  236. RNAi in bacteria - Why no finding for gene silencing in bacteria? (reply: 1)