bacterial transformation problem - (Dec/08/2011 )
I was doing a bacterial transformation with invitrogen omnimax cells
but i forgot to actually do the heat shock and the SOC medium before
i added the medium but did not do the incubation 37ºC, i went back and did the heat shock and the incubation on ice
its this a problem for the transformation efficiency?
-fpcc-
Probably, the conditions for the heat shock are timed based on the heating of a given volume for a specific time. They are also dependent on the concentrations of particular salts in the transformation buffer, which is why you should not exceed 5% of the competent cell volume for addition of DNA.
-bob1-
I dont understand w
fpcc on Thu Dec 8 23:45:14 2011 said:
I was doing a bacterial transformation with invitrogen omnimax cells
but i forgot to actually do the heat shock and the SOC medium before
i added the medium but did not do the incubation 37ºC, i went back and did the heat shock and the incubation on ice
its this a problem for the transformation efficiency?
I donot understand what you trying to ask. Theoretically what ever you did is completely wrong,But if you manage to get a clone you are lucky.
-Biouday-