What does the DH5alpha mean in E.coli DH5alpha bacteria - (Apr/26/2009 )
I'm using E coli DH5a for my transformations, and I was wondering what the DH5alpha stands for.
Is there anyone who can help me?
It is a strain name, much as E. coli O15H is a famous pathogenic strain.
bob1 on Apr 26 2009, 05:49 PM said:
I know it's a strain name, but I was interested if the D H 5a has some meaning. Some special features or something
The original reference is: Bethesda Research Laboratories. 1986. BRL pUC host: E. coli DH5α competent cells. Focus 8(2):9. (see here). DH5α was derived from E. coli DH5, described in Hanahan, D. 1985. in DNA Cloning: A Practical Approach. Glover, D. M. (ed.), Vol. 1, p. 109, IRL Press, McLean, Virginia. "DH" are the initials of Doug Hanahan.
HomeBrew on Apr 28 2009, 06:27 AM said:
Nice to be remembered for posterity by having a strain of bug named after you! Like good ole Dr Escherich! Could be worse, I suppose: you might have some horrible skin condition with your name on it!!!
HomeBrew on Apr 28 2009, 04:27 AM said:
Wow...Now I know DH5alpha is named from someone initials. Thanks.
Nice one Homebrew. I really appreciate that knowledge . D. Hanathan 5 ~~ this guy drives fast car and work late in the night mysteriously if i m not mistaken as written in Sambrook .
And all those highly competent E. coli cells you buy or make? He worked that out in D Hanahan. 1983. Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166(4):557-580, which, by the way, is also from where we get SOB and SOC media.
It was interesting to read all your answers
What could be its pathogenicity levels of this bacteria, is it BSL-2 pathogen? I was building a risk assessment form for this strain and came up with these questions.
DH5a and other common K-12 E. coli derivatives used in the lab are BSL-1, and non-pathogenic to normal healthy individuals. You should still use good lab practices in handling all bacterial samples, including autoclaving contaminated waste, bleaching discarded liquid cultures, and washing your hands.