Remove iron from agar? - (Nov/17/2010 )
Is it possible to remove iron from agar used to make plates? We currently use Chelex-100 to remove iron from our liquid cultures, but Im not sure if its possible to remove the iron from agar in order to make a defined iron source agar plate.
Use agarose rather than agar as a solidifying agent -- it's much more defined...
This paper might help:
J. L. Kadurugamuwa, H. Anwar, M. R. W. Brown, G. H. Shand, & K. H. Ward (1987): Media for Study of Growth Kinetics and Envelope Properties of Iron-Deprived Bacteria.
J. of Clinical Microbiology, 849-855.