bacterial growth - (Feb/26/2009 )
i have a very basic problem my e.coli cells(DH5alpha cells) had grown in lb with amp(100mg/l) but when i raised culture from the same fresh plate with half amp concentration there was no growth. this problem has been persistent . im nt able to find the answer.can anyone give me any suggestions plz?
thanks in advance
Was the antibiotic stock u used for both experiments (plates and culture) the same?
How old were the plates that you used?