how long for bacteria in 37 deg C? - (Oct/09/2013 )
my amp res plates after ligation dont have any colonies after overnight in 37 deg c, or have toooo small colonies, i wish to leave them longer in 37 to find more growth. how long can i leave the plates there before effects like plasmid loss and stuff happens?
If you don't have good colonies after 24hrs then your likely not going to get any. I wouldn't trust any colonies that appeared after that....
i agree with Missle. It's true. You can trust us on this one.