Dissolving Cholesterol in MSM Agar - (Mar/29/2011 )

Hello All!
I am trying to find a way to get cholesterol to break apart and dissolve evenly throughout my MSM agar medium. I know it doesn't have a high solubility, so I was thinking of using ethyl ether to dissolve it and use a TLC spray to spray the surface of MSM Agar to see if my bacteria can metabolize it. :huh: Thank you!

-rnazitto-

Hi,

Are you using bought cholesterol or have you extracted it from blood?

-swizla21-

swizla21 on Thu Apr 14 19:03:28 2011 said:

Hi,

Are you using bought cholesterol or have you extracted it from blood?

Bought cholesterol from Sigma

-rnazitto-