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Lac Promoter activity in P. aeruginosa - (Mar/10/2014 )



I want to create a plasmid that constitutively expresses a reporter gene in P. aeruginosa. Therefore I am looking for a constitutive promoter to insert into my reporter vector and according to this article ( the lac promoter (lacP) is a constitutive promoter in P. aeruginosa:


The lac promoter of E. coli acts as a constitutive
promoter in Pseudomonas spp. due to the absence of lac
repressor activity (Andersen et al., 1998).
I wanted to know if anyone had personal experience with P. aeruginosa and lacP who can affirm that it is indeed constitutively expressed in P. aeruginosa.


ptac promoter is a great promoter to use!