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Antibody Related Discussions
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problems with housekeeping antibodies when re-probing membranes - (reply: 3)
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Eliza and Antibody - (reply: 2)
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Lifespan of HRP-conjugated Secondary antibody @ Room temperature? - (reply: 2)
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Are hybridoma germline DNA and hyrbidoma cDNA sequences identical? - (reply: 2)
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how to select ki67 antibody - (reply: 1)
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The same strong bands with different primary antibody - (reply: 1)
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Secondary Antibody Problem - (reply: 2)
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monoclonal antibody immunization schedule - (reply: 1)
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Problem: mouse antibodies with mouse tissues - (reply: 4)
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loading control antibodies - (reply: 1)
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Diluting antibody stocks in glycerol - (reply: 8)
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Can we use ELISA capturing antibody for Western Blot Primary antibody? - (reply: 1)
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How divergent sequences is foreign enough for antibody production? - (reply: 1)
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Please help - FACS to ELISA for antibody testing - (reply: 3)
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Best phospho-ERK antibody - (reply: 3)
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Anti-his antibodies - Noob to protein work - (reply: 10)
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antibody concentration - (reply: 4)
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Costaining species-specific antibody question - (reply: 4)
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Secondary antibody overnight? - (reply: 5)
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Redoing only the Secondary Antibody staining on a Western - (reply: 1)
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Hybridoma cells not secreting antibody - (reply: 3)
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blocking and primary antibody in different buffers? - (reply: 1)
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Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody - (reply: 1)
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FACS antibody titration - (reply: 5)
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How to determine the optimal concentration of a primary antibody solution - (reply: 5)
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remove antibody from eluate in co-immunoprecipitation - (reply: 2)
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Huge background with HT22 cells phophoERK antibody - (reply: 3)
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Huge background with HT22 cells phophoERK antibody - (reply: 1)
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Polyclonal antibody production using SDS-PAGE gel fragment - (reply: 3)
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BSA versus milk in blocking and antibody incubation - (reply: 5)
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immunofluorescence using 3 antibodies like a sandwich - (reply: 3)
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Help on using different antibodies in the same membrane - (reply: 8)
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Amount of antibody used in Western Blot - (reply: 1)
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What if I froze a PE-conjugated antibody - (reply: 2)
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Sox-2 antibody detection using Western - (reply: 10)
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ChIP from mouse tissues with antibody raised in mouse - (reply: 1)
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Binding of reduced antibody to Protein-A?? - (reply: 1)
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anti-GLUT4 antibody, extracellular epitope? - (reply: 1)
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antibody in 40% glycerol - (reply: 6)
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How exactly does an IgG antibody control in ChIP work? - (reply: 3)
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Good anti mouse CD8 antibody? - (reply: 1)
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Storage buffer for antibodies - (reply: 3)
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Is it possible to raise an antibody against a 11aa peptide? - (reply: 2)
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Buy.Ask for buying some cell lines and antibodies - (reply: 3)
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Combination of secondary antibodies - (reply: 2)
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Incubation of Primary and Secondary Antibodies Help - Immunohistochemistry (reply: 1)
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Antibody Optimization - (reply: 2)
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Can i apply primary antibody for two nights instead of one? - (reply: 3)
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Monoclonal Antibody Conjugation - (reply: 1)
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choice of antibody - (reply: 3)
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GAPDH - Non-specific bands with GAPDH monoclonal antibody (reply: 1)
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What is happening to Antibody concentration after 48hrs? - Antibody concentration in rabbits after 48hrs. (reply: 1)
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HELP!- strange band appearing on immunoblot - strange band detected during blotting with anti-CTB antibodies (reply: 3)
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Growing hybridoma cells in medium without serum - (reply: 2)
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Antibody Affinity Purification - peptide column (reply: 2)
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Antibody vs Antigen concentration effect - (reply: 3)
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ELISA kits protocols, antibodies, control proteins, and more - (reply: 1)
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how to improve hybridoma production? - (reply: 3)
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OKT 8 antibody - (reply: 3)
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phospho akt and akt - does p-akt antibody recognize normal akt? (reply: 3)
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Using 2˚ antibody as a isotype control? - (reply: 4)
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help! hybridomas lose their function to produce Ab - (reply: 1)
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antibody dilution? - (reply: 1)
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antibody dilution? - (reply: 2)
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Western: Mouse antibody on mouse tissue homogenates - (reply: 3)
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Froze Dig Antibody (Roche) Now What? - (reply: 1)
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The Good Antibodies for Western Blots Thread - where people share info on antibodies that work well (reply: 2)
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why do my hybridomas seem to stop producing antibodies when screened 2 weeks lat - hybridoma culture (reply: 5)
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Immunoprecipitation and immunobloting with different antibodies - (reply: 6)
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Raising antibody for zebrafish - (reply: 2)
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Stripping Antibodies on Immunohistochemistry - (reply: 3)
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freezing antibody lyophilised - (reply: 2)
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anti-avidin antibodies in normal sera - high titers of anti-avidin antibodies in normal human sera (reply: 1)
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Concentration of fluorescent secondary antibody - (reply: 2)
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Invitrogen isotype specific secondary antibodies - (reply: 1)
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Primary Mouse antibody on mouse tissue - How to use mouse antibody to do western on mouse protein (reply: 2)
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neutralizing antibodies assay design - (reply: 2)
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Band at same position using different primary antibodies - (reply: 3)
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Untested Antibody for IHC - (reply: 6)
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antibody production-how to differentiate epitope as foreign - (reply: 4)
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Good Value Antibodies - (reply: 3)
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Western Blot Secondary Antibody Diluent - basic question (reply: 3)
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LC3 antibody advice - LC3 western blot (reply: 4)
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cell loss during antibody staining for flow cytometry - FACS staining protocol (reply: 5)
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Rabbit cells to grow bacteria in before immunization of rabbit for antibody prod - (reply: 2)
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Hybridoma culture problem. Help! - (reply: 2)
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Rabbit Polyclonal Antibody Production failed - (reply: 7)
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what is the best way to detect protein expression levels in plants - Also which company provides the best services on antibody production? (reply: 1)
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Antibody production. - Discrimination between almost identical proteins. (reply: 1)
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Antibody gene cloning - (reply: 3)
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B cell subset for hybridoma generation - Which B cell subset is used to make hybridomas (reply: 2)
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Antibody too much? - Signal not consistent, and fades quickly (reply: 5)
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Molar ratios of IgM and IgG antibodies in in vitro assays - (reply: 3)
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Antibody production in vitro - (reply: 4)
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Hybridoma maturation... is that possible? - (reply: 1)
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Basic antibody reactivity - confused understanding which antibodies would be suitable (reply: 3)
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Distinguishing antibodies by their light-chain type - (reply: 2)
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Sequential ChIP / ChIP-reChIP - Which of the two antibodies to use first? (reply: 1)
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anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
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IHC: antibody titration - determine the right dilution (reply: 4)
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Longer incubation in primary antibody - (reply: 2)
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about antibody Extinction Coefficient - (reply: 1)
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the specificity of HRP-conjugated antibody - (reply: 4)
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Monoclonal, biclonal, Triclonal.... ??? - (reply: 3)
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IGg or F(abī)2 Fragment of IgG - HRP-conjugated secondary antibody (reply: 1)
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Secondary antibody recovery - (reply: 4)
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Help with Twist antibody? - (reply: 1)
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Recombinant Antibody Production -Reducing / Non-reducing SDS page conditions - (reply: 2)
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primary antibody anti-human - mouse xenograft (reply: 1)
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Use of carbonate buffer to dilute antibody? - (reply: 4)
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IL-6 antibody - Which IL-6 antibody to use in Wb (reply: 2)
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antibody HRP - (reply: 2)
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How to test new homemade antibody? - (reply: 3)
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Murine antibodies - A panel of antibodies for murine blood cells (reply: 2)
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Determining whether the quality of an antibody and if it is species specific - (reply: 2)
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Immunofluorescence: polyclonal vs monoclonal antibodies and negative controls - (reply: 4)
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Is IgG monoclonal antibody better then IgM? why? - why everyone prefer IgG? (reply: 3)
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Concerning Cultivation of Monoclonal Antibodies - How long before antibodies spoil in media? (reply: 1)
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which elisa should i plan to determine the antibody concentration and specificit - (reply: 4)
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Custom Primary Antibody - (reply: 3)
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histology - help with antibodies (reply: 1)
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need advice about antibody characterization - (reply: 4)
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please recommend good anti-acetyl-histone H4 and H3 antibody - (reply: 3)
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Anti-idiotypic antibodies - (reply: 1)
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Antibody works in immunofluorescence, but not in WB - (reply: 4)
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using second antibody with a primary congugated ab - (reply: 1)
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antibody purification - ammonium sulfate precipitation - (reply: 7)
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wrongly used HRP-conjugated antibody in IP - what kind of result can I get (reply: 1)
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Optimising antibody concentration - hints and tips (reply: 1)
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Affinity/Avidity antibody Test - (reply: 2)
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IF with hybridoma supernatant - (reply: 2)
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Calculating concentrations of Antibodies when mixing - (reply: 6)
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Reviving a hybridoma from LN2 - (reply: 2)
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Phosphotyrosine antibody production - for Immunoprecipitation or affinity column (reply: 2)
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2 antibodies crossreacting with another protein - Immunology (reply: 2)
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PEG Precipitation of IgM Antibodies - (reply: 2)
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Antibody for Fusion Protein - Antibody/ies against fusion protein (reply: 2)
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Affinity purifed Polyclonal, better than monoclonal for IP? - (reply: 2)
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What is DME medium for Hybridoma cell culture - is DME medium and DMEM medium are the same for hybridoma culture?? (reply: 1)
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weak purified antibody - (reply: 4)
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What is the epitope of acetylated tubulin antibody? - (reply: 6)
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Western Blot Antibodies for inhibiting protein activity?? - (reply: 2)
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Is my antibody ChIP grade? - (reply: 10)
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HAHA (Human Anti-Human Antibodies) - Where to start? (reply: 1)
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Immunoprecipitation with two antibodies - (reply: 7)
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Western with conjugated antibody? - (reply: 2)
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primary antibody for western - (reply: 6)
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Cannot detect GFP using GFP antibody - (reply: 10)
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IgM quantification - Monoclonal Antibodies (reply: 1)
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Who can help me with my stupid mistake - wrong first antibody (reply: 3)
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How to design an antibody - (reply: 1)
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sample gives signal without capture antibody - No capture antibody but samples give high signal compared to blank (reply: 7)
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URGENT! use of a conjugate as a primary antibody - (reply: 2)
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Coupling magnetic beads-antibody - Pb in coupling antibody to beads (reply: 3)
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Good antibody for E. coli surface marker - phagocytosis assay (reply: 2)
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Sandwich ELISA - Sample values increases on dilution of coating antibody (reply: 1)
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Multiple antibodies give no signal - (reply: 10)
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Antibody Company Question - (reply: 3)
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problem with antibody blocking - (reply: 5)
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Hybridoma growth - (reply: 4)
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clon characterization - monoclonal antibody (reply: 2)
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How to detach an antibody from cell surface - (reply: 4)
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Tips for antibody purification from crude serum? - (reply: 3)
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monoclonl antibody application specifity - (reply: 1)
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labled antibody choice in elisa - (reply: 1)
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Genomic DNA Library - Problem of reactivity - Genomic DNA library screening with antibody (reply: 1)
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Secondary Antibody reacting with Loading Buffer? Is this possible? - Having problems with unidentified bands in western blot (reply: 3)
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how to get rabbit antibody for Western blotting - (reply: 2)
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Antibody stability if left out at RT over Xmas break? - (reply: 5)
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Do my antibodies need to stay sterile? - (reply: 5)
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Protein G vs Protein A for Rabbit Antibodies - (reply: 8)
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Expired conjugated Antibodies? - (reply: 4)
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anti flag anti his antibody - (reply: 2)
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Antibody compatibility between different species - (reply: 4)
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Custom antibody does not work as expect - (reply: 7)
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whole antiserum for indirect immunofluorescence? - (reply: 2)
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Mock IgG versus No Antibody - Negative Control - (reply: 6)
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Looking for endogenus expression by using custom antibody - (reply: 5)
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mammal model for human antibody production - (reply: 1)
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How topurify and measure stability of mAb IgM - (reply: 12)
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Antibody internalization - (reply: 1)
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3-colour labeling for the single argon-ion laser? - DNA + 2 antibodies (reply: 1)
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Looking for Abtibody Suppliers/Manufacturers - Hycridoma, monoclonal, manufacture, Antibody, mAb, ELISA, Western Blot (reply: 2)
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His tag antibodies - (reply: 1)
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Antibody staining - (reply: 6)
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Companies that synthesize peptide antibodies - (reply: 3)
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Primary antibody quantitation - (reply: 2)
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Antisera vs. Antibodies - (reply: 3)
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Laminin 5 antibody - (reply: 1)
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Suitable antibody for H3K4me3? - ab8580 and 07-473 both out of stock (reply: 4)
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nonreducing antibody in reducing condition - (reply: 1)
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Desmin antibody: muscle specific? - (reply: 1)
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seperating cells and antibodies - Separating cells and antibodies using centrifugation (reply: 2)
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Antibody Blues - Help (reply: 1)
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MBD2 and MBD3 ChIP - suitable antibodies needed (reply: 1)
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False positive from Endogenous Antibodies? - (reply: 1)
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Antibody titration and Cell number - (reply: 1)
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Non-specific antibody control for ChIP - (reply: 1)
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Blocking buffer for antibodies generated in goat. - (reply: 6)
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antibody differences - (reply: 1)
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stable transfection - V5-tag antibody (reply: 1)
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Can primary antibody be reused ? - (reply: 6)
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antibody crossreactivity - (reply: 1)
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What antibodies to use for ELISA?? - (reply: 2)
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DyLight vs. IRDye Western Blot Li-Cor Odyssey - Flourescent labled antibody DyLight vs IRDye (reply: 1)
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Annexin V staining in combination with other antibodies - (reply: 4)
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ELISA - how can i get Mab for NS1? (reply: 1)
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Antibody production - Tag removal prior to immunization (reply: 1)
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How to remove azide from antibody - (reply: 2)
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Primary Antibody for SPDEF - (reply: 1)
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sandwich ELISA (monoclonal and polyclonal matching) - (reply: 1)
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Primary antibody and Sodium azide - (reply: 2)
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primary antibody not working? - (reply: 1)
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Chimeric antibody Pepsin digestion - (reply: 1)
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Agarose-conjugated antibody for IP - (reply: 4)
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Antibody Validation - Please help me! THis is very Urgent (reply: 2)
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Monoclonal Antibodies - Hybridoma Screen (reply: 11)
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Primary antibody incubation length - (reply: 3)
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Ultrasonic bath for ChIP antibody incubation - (reply: 3)
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Antibody Characterization - KO vs. WT or Heterologous overexpression (reply: 8)
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antibody labelling - not enough Ab (reply: 2)
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Antibody coupling to Magnetic Beads - (reply: 3)
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antibody production..does it matter if the protein is denatured or not? - my fragments are insoluble :( (reply: 3)
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mAb purification but precipitated - (reply: 13)
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How to store membrane with primary antibody? - (reply: 5)
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DRG MARKER - dorsal root ganglion antibody marker (reply: 1)
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Blocking buffer as antibody dilutent? - (reply: 4)
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antibody interaction - (reply: 1)
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Anti-FLAG antibody help - Troubleshooting for Sigma anti-FLAG (A8592) (reply: 3)
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Antibody to mouse antigen raised in mice? How it is working? - (reply: 10)
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Antibody titration - (reply: 5)
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Secondary Antibody: adsorbed? - (reply: 1)
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wash blots between antibody incubations with TB-'SALINE' pH 7.4 - why saline.. why this pH? (reply: 1)
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uncertain about stable overexpression - discrepancy between different antibodies (reply: 5)
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Out of stock: trimethyl H3K4 antibody for ChIP - (reply: 5)
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Chip problem: antibody or cell number?? - (reply: 6)
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Finding antibodies - (reply: 6)
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antibody for immunocytochemistry - only validated for Western-will it work? (reply: 4)
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Collagen 1 - Long shot, looking for an antibody (reply: 1)
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How to check antibodies for viruses - (reply: 1)
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fungi in antibody working solution - can I filter and re-use? (reply: 2)
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Sumo-1 antibody - (reply: 1)
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if antibody solution could be used again? - (reply: 8)
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Streptococcus pneumoniae antibody - (reply: 1)
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antibodies for immunology - antibodies (reply: 1)
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error in storage temperature of antibodies - (reply: 4)
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ecdysone antibodies - (reply: 3)
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Tips on re-use of primary antibody in Western blot - (reply: 14)
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Too cold for agarose beads to survive? - Antibody Affinity Purification (reply: 5)
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High background with FLAG antibody - (reply: 1)
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Determine antibody concentration - (reply: 12)
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Antibody storage - (reply: 10)
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IP problem: antibody binds too few antigen - (reply: 13)
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Multiple antibody blotting - western blot - (reply: 16)
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recommendation needed for a good p53 antibody - (reply: 2)
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AP-2 mu-2 subunit antibody - (reply: 2)
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IgM hybridoma protocol - Protocol targeted for isolation of IgM secreting hybridomas (reply: 5)