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How to check an antibody is ChIP grade? - (May/17/2013 )

how do I check that my antibody is ChIP grade?

Hi all,

I preforming a ChIP experiment, and to check the efficiency of my antibody, I will make a western blot after IP, but...... It is necesary to reverse the corss linking before?



I just do a titration against my endogenous protein, overexpressing and knockdown lines. I also like to compare my results against any qPCR data I have.