Best phospho-ERK antibody - (Feb/28/2012 )

Hi
I want to ask you what is the best anti-phospho ERK1/2 antibody for western blot. I ussually used one from Sigma (cataloge number: 9692), but it is just doesn´t working anymore.

Like I heed to all the steps of my protocol, I think i have a problem with the antibody, and that is why I would like to know what is the best antibody for detect activated diphosphorylated ERK1/2

PD: i work with mouse T cells lysates.

Thanks!

The rabbit monoclonals from Cell Signaling are excellent. Try one of these:

http://www.cellsignal.com/products/4370.html
http://www.cellsignal.com/products/4377.html
http://www.cellsignal.com/products/4376.html

They all indicate they detect dually phosphorylated ERK1/2 and also singly phosphorylated ERK1/2 (either the Thr or the Tyr depending on the antibody). This was likely the case with your Sigma antibody as well.

doxorubicin on Wed Feb 29 12:37:16 2012 said:

Try this one http://www.scbt.com/datasheet-16982-p-erk-1-2-thr-202-tyr-204-antibody.html
Way cheaper than Cell Signaling and it works great!