Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE and Western Blotting

Secondary antibody recovery - (Oct/22/2010 )

Hi.

As (I believe) the majority of the people do, I re-use primary antibodies. Either if they are diluted in milk or BSA I store them at -20C. On the other hand, in my previous lab, we never re-used the diluted secondary(+HRP) antibody. But in my new lab I am short of secondary Ab and therefore I would like to know if it is possible to re-use it.


Thanks!
:)

-cardosopedro-

yes you can, but not in -20C. It is recommended to keep at 4C if you dilute your antibody in TBST with milk.
I even re-use my primary though the concentration drops every time I use

-Curtis-

Curtis on Sat Oct 23 14:16:56 2010 said:


yes you can, but not in -20C. It is recommended to keep at 4C if you dilute your antibody in TBST with milk.
I even re-use my primary though the concentration drops every time I use


Thanks.

Oops, I kept it at -20 C on Friday. Do you think it will still work?

-cardosopedro-

cardosopedro on Sun Oct 24 11:15:14 2010 said:


Curtis on Sat Oct 23 14:16:56 2010 said:


yes you can, but not in -20C. It is recommended to keep at 4C if you dilute your antibody in TBST with milk.
I even re-use my primary though the concentration drops every time I use


Thanks.

Oops, I kept it at -20 C on Friday. Do you think it will still work?

Hola,Waiting the answer of your first adviser, I reuse both 1 and 2 abs and I store them at -20 after each use, only I taw them in a water bath at room temperature. Good luck

-protolder-

Thank you for the answers.

-cardosopedro-