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Check if an antibody is internalized - (Oct/03/2013 )

I want to check if an specific antibody is internalized into the prostate cell line through its specific antigen., I need to help provide me with the reliable and acceptable method with scientific justification.,

 

my lab has core facility including IF, Western blot, Eliza

 

Thankful for all help

Nejadmoghaddam

-Mohammad Reza Nejadmoghadd-

Is standard immunofluorescence not enough?

-JYaron-