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Help with Twist antibody? - (Oct/20/2010 )

I am currently working on the oncogen Twist. I used to use the Twist polyclonal antibody from Cell Signal and everything works fine.
But sad thing is they don't sell this antibody anymore and I ran out all of my stock.
I bought the Santa Cruz sc-15393 but the result is so horrible: no signal at all.~~~><||||
I also tried Twist antibody from Abcam, also not good for Western blot.

My work is on the edge to go for publication and right now stuck here. Q_Q

Is there anyone would like to recommand a good Twist antibody?



Hi There,

Greetings from Santa Cruz.

I see you are having trouble using your Twist antibody for Western Blot. Did you already contact Technical Support here at Santa Cruz? We warranty all our products for 1 year from the date of purchase for the species and applications listed. We would be glad to help you try to get this WB to give you signal. If you want to contact us and share your protocol and results with us, we would be glad to offer suggestions to your protocol for this antibody or if we see necessary, we can replace it with a different lot (if we have one available) or another Twist antibody.

Please feel free to give us a call or send us an email and we will be glad to help you. You can reach us in several ways:
Toll Free: 1.800.457.3801 ext. 2

Good luck in your research!


Santa Cruz Technical Support Team

Santa Cruz Biotechnology,
Phone: +1-800-457-3801 ext. 2
Fax: +1-831-457-6013

-SCBT Tech Support-