Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE and Western Blotting

Recommend anti-rat HRP secondary antibody - (Oct/01/2013 )



could you please recommend a sensitive but celan anti-rat secondary antibody for western blotting (conjugated to HRP)?


Thank you!




Hi.  I use Jackson ImmunoResearch secondary antibodies.  They are very good and resonably priced.  Just make sure you get the ones that are 'minimally cross reactive' to other species.  As long as I've gotten the min. cross secondaries, I haven't had background issues.


I'm using this one and got always good results and no/low background.