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Multiple bands on blot with one antibody - (Aug/06/2012 )

Ran a standard blot and saw 2 bands with a ~10kD difference (e.g. 125 and 130kD). Was wondering how do we know which band is the 'true' band? and is it common to see more than one band?

-science noob-

yes it's quite common to see non-specific bands. You just need to run controls to know which band is the true band.