Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE and Western Blotting

Western with conjugated antibody? - (Mar/02/2010 )

Hi all,
Is it possible to use primary antibody, which is conjugated to fluorescent label, in Western blot with secondary-HRP conjugated antibody?
Thanks for answering!

-katenkak-

sure, as long as the secondary is against the primary antibody.

-mdfenko-

Thank you! Have a nice day!

mdfenko on Mar 2 2010, 10:14 PM said:

sure, as long as the secondary is against the primary antibody.

-katenkak-