Distinguishing antibodies by their light-chain type - (Nov/20/2010 )
Hello dear colleagues,
Is there any easy way to separate antibodies (particularly, IgG) based on light-chain typing (lambda or kappa)?
Thanks a lot!
In an immunoassay using kappa or lambda specific secondary antibodies.
I think you mean purify not quantitate? I believe you would first isolate all the IgG then run a column against the specific light chain you are intersted in thus your product would be IgG kappa or IgG lambda.