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Distinguishing antibodies by their light-chain type - (Nov/20/2010 )

Hello dear colleagues,

Is there any easy way to separate antibodies (particularly, IgG) based on light-chain typing (lambda or kappa)?
Thanks a lot!


In an immunoassay using kappa or lambda specific secondary antibodies.


I think you mean purify not quantitate? I believe you would first isolate all the IgG then run a column against the specific light chain you are intersted in thus your product would be IgG kappa or IgG lambda.