antibody dilution? - (Apr/06/2011 )
my protocol I have been given says to use the antibody against my surface protein of interest at a final concentration of 10ug/ml in 1ml of media for live cell staining.
Given that the antibody is supplied in a vial labelled 100ug total, this seems an awful lot of antibody for 1 slide, am I missing something here in my calculations or do I really have to use 1 vial per 10 slides?
I am thinking you can re-use the solution for more than one slide. Otherwise your calculation is (sadly) correct. 100 ug is not a lot.