Polyclonal antibody precipitating - (Nov/28/2013 )
I received a custom rabbit polyclonal purified antibodies (3 in total) from Thermo Fisher. When I first received them, they worked great, now there is a precipitate and the binding is decreasing for the antibody. The customer service rep told me I can try adding 0.5-1.05% BSA, but that seemed to have little effect. I have also heard of using potassium salt, but I am not familiar with this method to regain antibody activity. Can anyone give me any suggestions? Thanks.
I'm sorry to say that, in my experience, there is no getting the antibody to 'regain activity'. You can try a few things to prevent any more of it from precipitating. Has it been stored at 4C or -20C?