What sencondary for Chimera Fc primary antibody? - (Apr/07/2014 )
I got hold of a Chimera Fc primary antibody that I would like to use it for WB and IHC. However I can't figure out what sencondary I should use. The Data Sheet says the antibody was produced in a mouse myeloma cell line (NS0-derived). None of the citated papers used it for WB or IHC.
You probably need an anti-mouse IgG secondary Ab.
Could you upload that data sheet?
here the data sheet http://www.rndsystems.com/pdf/796-ic.pdf
According to the data sheet, you should use anti-Human IgG secondary Ab.
Also, your RnD product is not an antibody, its a fusion between your protein (ICAM) and Fc of Human IgG.
I see... now everything makes sense. So it is not an antibody-antigen situation but a ligand-receptor.
Thanks you so much tkf!