Anti-Guinea Pig IFN-g and TNF-a - Antibody Suggestions?! - (Mar/12/2014 )
I am having trouble finding flow antibodies to look at IFN-gamma and TNF-alpha in guinea pig samples. So far I've found that Novus has an ab for TNF-alpha with cross-reactivity in guinea pigs, but that's pretty much it. I was wondering if anyone had prior experience or suggestions. I'm not picky about the fluorophore these antibodies are conjugated to.
They are distantly related to rats and mice, so I would try antibodies against those species. The major problem is that guinea pigs aren't all that common as a lab animal for these sorts of studies (more used for physiology/anatomy and sociology), so finding antibodies that have been tested will be difficult.
Yes, it's difficult to find an AB that has been tested for flow cytometry.
We offer a free trial size antibody for evaluation if you are interested in testing one of our TNF-alpha antibodies in FC.
Please visit St John's Laboratory website http://www.stjohnslabs.com/services/Premier-Partners-Scheme
We only have 1 IFN gamma antibody, but a lot more TNF alpha. If you are outside the UK, we will need to charge shipping
Hope this helps