GAPDH - Non-specific bands with GAPDH monoclonal antibody (Jul/18/2011 )
Hi every one. I have used monoclonal antibody to GAPDH and detected a non-specific band at about 100 kd. Has anybody else ever got that? The antibody I am using is one of abcam's. Thanks.
I think you will find that everyone who has done westerns will at some point have had non-specific bands... There are lots and lots of threads on here with information on how to reduce non-specific binding.