Start codon after promoter - (May/22/2014 )
How far can you put the start codon (ATG codon) after the promoter? Is there a general rule, I am guessing its best that the ATG codon is as close as possible to the promoter sequence or does it not matter that much?
Can I have , for example 100 basepairs between the end of the promoter and the ATG codon?
thanks in advance
I think 100 bp will be fine. If I remember the little I know about this topic correctly, then some mammalian promoters are several hundred bp 5' of the ATG.