ClaI restriction enzyme efficency - (Jun/19/2014 )
I am going to start a cloning and I am considering to use the ClaI restriction site for insertion of a fragment into my vector. I know that ClaI can be methylation sensitive depending on the the surrounding sequence. the nucleotides around the site in my vector are CATCGATA so I think it will not be methylated, still I have not worked with ClaI before so I was wondering if it is an easy enzyme to work with and also if there are other issues that I need to consider about this enzyme.
I've had no particular problems with it in my limited uses.