Maximum recovery time after electroporation - (Aug/21/2009 )
Does anyone know what's the maximum recovery time after electroporation? I use Methylobacterium extorquens and we usually use 24 hours and it works well. But often I have to come back on saturday to plate my tranformation. It's why I'm asking the maximum recovery time, because if I can incubate the cells for 48 hours it will be good for my personnal life!!
The cells are in 1ml Minimal Media + Methanol. I didn't think about incubation at room temp! Great idea, I'm going to try that.