What are "Ella-cre" mice? - (Mar/21/2010 )
I'm reading an article where they engineer knockin transgenic mice.
At one step they inserted a DNA sequence containing Neomycin-resistance into a replacement vector. Then they injected this knockin vector to embryonic stem cells, then they injected these into mouse blastocyst. Fine so far.
Then, they cross-bred these new mice with Ella-cre mice in order to get rid of the Neo-resistance cassette.
OK, I understand this but I want to know what are these "Ella-cre" mice? I searched around and couldn't find.
have a look for cre-lox system.
E2A-Cre mouse express Cre from oozyte stage onwards, and in all cell types. It is thus used to achieve recombination in all cells (Deleter-Cre).