hENT1 - (Jul/18/2010 )

hi all

Iam trying to clone hENT1 protein into DH5 alpha, but hasnt any success for many weeks from now. Even if I get some colonies, I dont see the bands in the gel with the original size I needed, but a product less than that. Does any one know that if this protein is toxic to bacteria, so that it throws the plasmid out. Any help will be appreciated.

jaya

Okay. Lets start by establishing a few basic facts.

Is the hENT1 gene under expression of a prokaryotic promoter?

Could you share how you when about cloning this gene. Please include every detail. Did you gel purify the insert? Where are you getting the hENT1 gene from?