dephosphorylate sticky-ended vector - is it necessary? (Jul/14/2011 )

Hi,

Currently I'm doing sticky-end ligation. I cut my vector and PCR product (with BamHI overhang) with BamHI.

So, is it necessary to dephosphorylate my vector to prevent self ligation?

Yes ofcourse, you need to dephosphorylate the vector....

badguy on Fri Jul 15 03:53:11 2011 said:

will my insert self ligate after treatment with BamHI?