Cloning of IL8-promoter - (Nov/18/2011 )
I am new to molecular biology and I just started following this class.
However we now have a project on the cloning of the IL-8 promoter out of pDD-Zsgreen1 Reporter plasmid and try to place this into pGL3-Basic Vector.
Now I have 2 problems:
I have to find out, how to digest/cut the IL-8 promoter out of pDD-Zsgreen1. I cannot find the IL-8 promoter sequence and I cannot find the right restriction enzyme to cut this promoter.
Does anybody know where I can find the sequence of the IL-8 promoter, so that I can find the right enyzme to cut this promoter?
My overal knowledge about Molecular Cloning is very low. Does anybody know a book or website where I can read upon this subject, so that I can make a flying start on the project?
Thanks in advance,
I really need to know these things!
to find the human IL8 promoter
try here: http://www.ensembl.org/index.html
or there: http://genome.ucsc.edu/cgi-bin/hgGateway.
Do you have the complete sequence of your plamid?
Hey, thanks! i'll take a look at it.
In fact, I dont have the complete sequence of my plasmid yet.
Could I find it somewhere on the internet? I tried 'googeling' it, but couldn't find anything.
Google is always a good start. Do you have a "map" of the plasmid, or the correct full name used in publication?