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For E. coli translation of an mRNA, do you need space between the stop codon and - (Nov/03/2011 )

I am trying to design a forward primer to amplify an ORF to make an mRNA to express a protein without the fusion tags in a bacterial system. So the vector goes something like this

*<9-10 nucleotides>.

My question is: Do I need to add any spacer nucleotides between the first stop codon and the next ribosome binding sequence (at the *), or can they be right next to each other?

-Brian Jackson-

can be right after each other ...e.g. see the lac operon!

so no special spacer needed!



Great, thanks!

-Brian Jackson-