For E. coli translation of an mRNA, do you need space between the stop codon and - (Nov/03/2011 )
I am trying to design a forward primer to amplify an ORF to make an mRNA to express a protein without the fusion tags in a bacterial system. So the vector goes something like this
My question is: Do I need to add any spacer nucleotides between the first stop codon and the next ribosome binding sequence (at the *), or can they be right next to each other?
can be right after each other ...e.g. see the lac operon!
so no special spacer needed!