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Western blotting Related Discussions
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secondary Ab for immunofluorescent staining - secondary Ab designed for WB was used for IHC (reply: 5)
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Unidentified problem with low concentrated samples - Variable limit of detection of my western (reply: 9)
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Strange aspect of bands in Western Blot - (reply: 2)
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Reason of Cross-reactions in Western blot? - without specific band (reply: 5)
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optimal conditions for wet transfer in western blotting - (reply: 3)
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WB vs Immunostaining - (reply: 1)
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Non-specific bands with secondary Antibody on Western Blotting - (reply: 1)
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Monoclonal Ab Western and ELISA issue - (reply: 2)
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western blot from heart lysates - membrane extractions too (reply: 2)
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protease inhibitor, TRI reagent and western - (reply: 2)
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wobbly bands in western blotting - (reply: 5)
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WESTERN TRANSFER PROBLEM - (reply: 6)
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From Western To ELISA - HELP!? - Are my parameters acceptable for use in ELISA? (reply: 4)
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Substrate for AP conjugated secondary antibody - is it the same as Western blot? by NBT and BCIP? (reply: 2)
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Experimental design question for Western blot - (reply: 3)
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How to release antibodies from beads after IP? - in order to run ELISA after IP, not Western Blot. (reply: 7)
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beta actin vs GAPDH as loading controls for Western blotting - (reply: 1)
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Western blotting with blood serum samples - (reply: 2)
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My first western....advice please - (reply: 3)
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Non-denaturing western blot - I need a transfer protocol for non-denaturing gel (reply: 1)
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Western Blotting help - (reply: 1)
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Cytochrome C detection by Western Blot - a typical question (reply: 2)
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Nuclear fraction marker for Western Blots - (reply: 4)
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No bands observed on anti-His western blot - (reply: 11)
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How to reduce amount of antibodies used in WB - (reply: 4)
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unexpected western blot band - western blot (reply: 1)
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Western for a 500kda protein - some troubles, need help! - (reply: 1)
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Western blot of the supernatant - (reply: 5)
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Supernatant for western - (reply: 3)
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CN staining in Western blot? - (reply: 2)
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What high molecular weight loading control for Western blotting? - (reply: 3)
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problem with transfer during western blot - western blot /sds page (reply: 2)
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Which quantity of purified protein in WB? - (reply: 2)
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Collagen bands in Western Blot - (reply: 2)
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Western blotting troubleshoot - (reply: 1)
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Inconsistent Western blotting results - (reply: 3)
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western blot normalization - (reply: 1)
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Vertical streaks on WB following IP - (reply: 3)
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DMSO in western lysis buffer - (reply: 1)
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non-specific bands in western blotting - (reply: 3)
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integrin beta1 western - (reply: 1)
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combining the results from different gels in western blot - (reply: 3)
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PVY western - (reply: 1)
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western blot problem - no expression on stable transfection. (reply: 9)
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Urea and Western Blot - (reply: 4)
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mouse and rabbit antibodies in western blot - (reply: 2)
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High Molecular weight reference for Western. Help! - (reply: 7)
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Used western blot film-how to do stripping? - (reply: 1)
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western blot--protein concetration - too dilute?? (reply: 3)
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how to see A beta by western blot - (reply: 6)
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Western Blot of Bacteria - Need some advice on performing a Western Blot from Bacteria (reply: 3)
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Western blotting for Histones - (reply: 1)
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what is the equation for quantitative western blot - (reply: 3)
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Cell lysis method for running cell lysate on Western blot - (reply: 4)
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How to choose the right buffer for my western blotting? - (reply: 2)
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Stripping for Western blots - What is the common procedure for stripping? (reply: 4)
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western transfer efficiency.. with or without methanol / SDS? - any differences? for what kindof proteins? (reply: 1)
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western blot current setting.. for 1 or 2 blots together? - (reply: 1)
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A basic Western blot question - (reply: 2)
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Western Blot for weakly expressed protein - (reply: 3)
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why need to do protein assay before western blot? - (reply: 3)
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which internal control for my western blot? - (reply: 6)
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western blotting loading control - (reply: 4)
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High background on westerns - (reply: 5)
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WB reverse image solution?! - white band on darck background (reply: 2)
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Western vs. ELISA - (reply: 5)
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Storing protein extracted from tissues for Western blot - (reply: 9)
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different western results when using acetone prep vs crude protein - (reply: 3)
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help, :-( Actin completely blank on western blot - (reply: 6)
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Which p-Serine antibody is suitable for IP and WB - (reply: 1)
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How can I load equal amounts of protein into each well during SDS-PAGE? - SDS-PAGE, WB, BCA assay, Actin (reply: 7)
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Collagen I and V western blot - Blue Native PAGE - Blue Native PAGE (reply: 7)
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3 bands on WB instead of 1 - (reply: 4)
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Real time PCR - RT-PCR vs Western blot (reply: 8)
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antibody incubation on western blots - difference between 4°C and RT (reply: 10)
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western blotting - for longer exposures (reply: 1)
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My antibody does not work for IP, but works well fpr western? - antibofy not working for IP (reply: 12)
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Sponge pads in semi-dry westernblotting? - to save Whatman paper... (reply: 3)
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WB anti-caveoline1 not reproducible - (reply: 2)
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Problems with HMW proteins and Western blot - (reply: 5)
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how to prepare blood sample for western blotting? - (reply: 3)
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Nitrocellulose or PVDF membrane for western blot - which one???? (reply: 8)
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Preparation of Cell Lysate for Western Blot - What did I do wrong? (reply: 14)
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Western kit for human samples? - (reply: 1)
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Western Blot/SDS-PAGE samples - boiled or not boiled? - (reply: 2)
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can detet anything with my western membrane - (reply: 8)
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Detecting Transcription factors in western blot - Hard time detecting LEF-1(TFs) (reply: 2)
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western background problem - (reply: 3)
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WESTERN BLOT : upper band at 45 kDa in blot of cytochrome c - (reply: 2)
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WB trouble - no signal of low kd protein - (reply: 5)
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Confirming IP with WB - Can I use the same antibody for both IP and WB? (reply: 5)
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Special procedures for phosphorylated proteins? - During isolation and western blot (reply: 3)
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Western Blot of cells in Laemmil - (reply: 5)
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Serial Dilution and Western Question - (reply: 5)
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Western Blot: Re-use of membrane? - (reply: 4)
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extract proteins for WB - (reply: 4)
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for western blot - (reply: 1)
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Western Blotting problem - p21 detection in MCF7 lysates (reply: 2)
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non-specific binding in western blotting - non-specific binding in western blotting (reply: 7)
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Yeast protein extract preparation for a quick western blot - (reply: 5)
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which is more sensitive: IP or western blot? - Looking for transcription factor - would IP help? (reply: 5)
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Western Blot Hollow "Ghost" Bands with ECF - (reply: 1)
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peptide expresion, but no western blot bands. - (reply: 2)
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Very odd western bands - (reply: 4)
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western blot for phosphorylated proteins - (reply: 3)
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cathepsin C detection in Western blot - (reply: 1)
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western blot transfer debate - (reply: 5)
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westerns, protein concentr and RIPA buffer - (reply: 2)
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Oligodendrocyte's and tubulin antibody in Western Blot - (reply: 1)
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western blot for nuclear and cytoplasm seperation sample - (reply: 3)
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Why 2 bands for housekeeping? - western blot (reply: 2)
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getting rid of nonspecific background in westerns - (reply: 3)
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How to get rid of non-specific bands on Western blots. - (reply: 9)
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western blotting transfer onto membrane:why does current increase? - At constant voltage, resistance increases. The current also increases. (reply: 5)
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WB - Wet - Markers not transferring? - (reply: 2)
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Western Blot of myosin heavy chain - (reply: 3)
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WB: protein amount/ lane ? - What is the optimal protein amount per lane in WB ? (reply: 6)
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Protein Extraction from cell media for western blot - (reply: 3)
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Western problem : no signal - could that be linked to pH? (reply: 2)
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Western blot problem--- bar shape band - (reply: 5)
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c-Myc antibody for Western - (reply: 1)
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problem on western blot - (reply: 9)
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Transfer during western blotting - (reply: 8)
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ELISA antibody-use for WB? - (reply: 6)
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Western blot: Circular bands - artefact? - (reply: 4)
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WB for secreted protein? - (reply: 5)
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Western Blot Troubles - (reply: 10)
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Western Blotting using Anti α Tubulin antibody: Bands too high! - (reply: 3)
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re-probing western blot with chemi-luminiscent substrate - (reply: 2)
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collagen western blotting - (reply: 1)
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Fluorescence detection of Western blots - (reply: 3)
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WB advice - (reply: 2)
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Advice to improve westerns - looking for ideas to clean up my WB results (reply: 13)
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MW marker for 150 kDa protein in Western blot - (reply: 5)
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Humid and semi-dry Western blot - (reply: 1)
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Western blots - positive control - (reply: 2)
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loading for western blot - (reply: 7)
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Native PAGE and WB - (reply: 1)
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western blotting for cytochrome c - (reply: 4)
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WB quick question - (reply: 8)
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loading controls for western. which one to choose? - (reply: 5)
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Difference bwtween BME and DTT in sample buffer for WB after IP - (reply: 10)
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subcellular fractionation confirmed by western not consistent with microscopy - nuclear protein by microscopy not nuclear in fractionated sample -WHY? (reply: 3)
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STAT-3/phosphoSTAT-3 western blot - (reply: 6)
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Western blot sample storage - (reply: 2)
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How to interpret a Western Blot? - (reply: 5)
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western blot protocol - (reply: 1)
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adhesion molecules by westerns - (reply: 3)
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western with nuclear extract - does high salt/glycerol affect running (reply: 2)
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reducing and non-reducing condition in westernblot - (reply: 4)
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Western Blot- - Bands appear only nc eon the film! (reply: 3)
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how to evaluate the siRNA silence efficiency? - western or rtPCR (reply: 6)
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Storing a western blot over the weekend - (reply: 4)
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Knockdown detected by western but not RT-PCR - (reply: 3)
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What is west western blotting? - Want to know the details and difference between western blot and west (reply: 2)
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Actin bands not equal on Western blot - (reply: 5)
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protein complex purification, western shows tag protein was pulled out, but silv - (reply: 10)
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Western Transfer and Detection of small protein - (reply: 6)
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anti-BRCA1 (ab-1) for WB - (reply: 8)
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black dot blackground on western - (reply: 7)
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problem with western blot - (reply: 7)
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Western Blot Troubleshooting - Marker fading and other issues (reply: 7)
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Strange phenomenon with western blot - Double bands (reply: 2)
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problem in western blotting - (reply: 5)
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western to detect ATM - (reply: 3)
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Western Blot Feedback - (reply: 1)
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white bands in wb - (reply: 4)
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Many negative bands in WB - (reply: 4)
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western vs elisa - (reply: 4)
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TRANSFER (WESTERN BLOT) - Transfer Tips (reply: 25)
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Antibody from short peptides - Time saving, but do they work for both West and ELISAs (reply: 4)
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Need help with WB/IP sensitivity issues - As in way too sensitive! (reply: 3)
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Urgent stuff about western blotting - (reply: 9)
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western blot problem - mailed by baatrix (reply: 7)
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Need help! Problems on Western blotting - (reply: 7)
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WB for comparing the level of certain protein in two different cell lines - (reply: 2)
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about western blot - Please help me (reply: 12)
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cell culture western blot - (reply: 15)
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failure with first western blot - (reply: 8)
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G protein coupled receptor - western blot - (reply: 3)
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Western Blot - unspecific antibody binding - (reply: 9)
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reducing background in western... - ...by blocking the antibodies (reply: 2)
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My ChIP product Western is dirty - (reply: 7)
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Why milk is used in Western blotting? - (reply: 29)
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Questions about phospho-protein level in western blotting - (reply: 5)
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can we purify the protein from westernblot membrane? - (reply: 5)
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Western Detection of Transcription Factors - -more difficult than other proteins? (reply: 3)
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Is Western blot really quantitative? - (reply: 8)
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protein preparation for Western Blot - (reply: 6)
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Question about high salt TBST in WB - (reply: 1)
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Western Blot on an IEF separated recombinant protein - (reply: 5)
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can u give ur opinion in my western blot results? - (reply: 15)
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Preparing blocking solution for Western Blotting - what milk power to use? - western blot (reply: 11)
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serum samples for western - (reply: 7)
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ABout the solution in Western blot and SDS? - (reply: 5)
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Western blotting -smearing effect - (reply: 6)
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high backgroud on western - (reply: 2)
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Multiple bands on Western blot for an expressed protein - (reply: 7)
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AT1 receptor expression and quantification (WB) - need advice (reply: 1)
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Western blotting - How to interprete (reply: 2)
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Anti-FLAG antibody working for Western blot - I unsucessfully tried many different (reply: 4)
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Western blotting for ER - problems - (reply: 7)
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Anti-PolyHistidin antibody - Best His6 antibody for western (reply: 2)
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SDS-PAGE over heating and will western work from that gel? - sds page running and western blot of gel run under too much heating (reply: 9)
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western blot milk dots - (reply: 10)
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calculations of western bands - which software is better? (reply: 3)
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problems with Immunoprecipitation- Western Blotting - Rabbit IgG secondary givi - (reply: 1)
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my protein is 18Kda but runs as a 25kda on WB - why ? - (reply: 13)
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How to increase transfer efficiency for Western blot - (reply: 9)
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nuclei fractionation for western blotting - (reply: 1)
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help me ! dying about my western blot - (reply: 14)
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Western Blotting detection Antibodies........... - Are anti-sheep and anti-goat HRP interchangable? (reply: 2)
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Western Blot Analysis - Technique for Protein determination (reply: 6)
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"Ponceau S" distorting the bands in western blot? - (reply: 5)
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phosphorylated protein Western blotting - HELP!! (reply: 10)
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Immunofluorescence microscopy, Western Blot, ELISA, ... - (reply: 4)
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Okay to use different exposure times to quantitate a western? - (reply: 1)
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How to strip PVDF membrane after imaging ? - For western blot (reply: 2)
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how to prepare total cell lysate for Western blot? - (reply: 10)
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Who sells good primary antibodies for Westerns? - Who's your favourite supplier? Least favourite? (reply: 24)
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One Antibody in Western Blot - (reply: 4)
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Extra band in western blot - (reply: 1)
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Harvest of cells from 96 well plates for Western blots - (reply: 8)
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quantification of WB bands- how to get similar values from different experiments - (reply: 4)
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DsRed fusion protein not glowing - It is there by WB though (reply: 3)
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Western blot bands fade out - (reply: 9)
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Problem with western blot with VEGF receptor 1 and 2 (ab2350 and ab2349) - (reply: 1)
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Not getting actin bands equal on Western blot - (reply: 11)
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Western after Ip- no IP protein, but .... - (reply: 1)
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Western Blot - membrane-bound protein boiling step - (reply: 1)
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What is Far Western Screening - (reply: 3)
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Better resolution on SDS-PAGE then Western.... - (reply: 2)
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Western blotting without transfering protein to membrane - (reply: 4)
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Western AB unselective or posttranslational modifications? - (reply: 1)
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no reference in the westerns blot (like actin) - are these results publishable? (reply: 7)
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I have problem on Actin band - SDS-PAGE and Western blot (reply: 3)
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how to make neutrophil cell lysates for Western blot? - (reply: 3)
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Western Blot on an IEF separated recombinant protein - (reply: 6)
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milk in western blotting - (reply: 2)
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Why two bands in my Western blotting - (reply: 1)
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western blot ECL vs fluorescence - (reply: 2)
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normalizing for loading and transfer error during western blot - (reply: 4)
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Western Blotting substrate - Lumi-Light Western Blotting Substrate (reply: 4)
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Non specific bands on Western blot - (reply: 1)
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Problem of spontaneous MAPK detection from Western Bolt - (reply: 9)
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Normalizing Western Blot to cell number without cell counting - (reply: 5)
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western blot looks strange - western blot looks very weird (reply: 5)
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western after BN-page - (reply: 6)
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Protein yeast extraction for Western blot - (reply: 3)
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An annoying Western blotting problem - (reply: 3)
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Western blooting interenal control problem - Protein and proteomic (reply: 3)
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western blot - missing expected band - (reply: 1)
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Washes for Western, PBS or TBST? - (reply: 12)
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Two antibodies on one western Blot? - (reply: 4)
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Western blot problems... - Western blot: Bad blotting? (reply: 7)
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Loss of signal during film exposure western blot - (reply: 1)
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many bands other than my protein on western... - please help me to explain this... (reply: 5)
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membrane protein western blot - (reply: 2)
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westernblot problem! - membrane protein! (reply: 2)
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Question on stripping westerns... - (reply: 5)
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How many cells to lyse for Western? - Decreasing signal in my Western Blot (reply: 2)
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detection of phosphorylated proteins on WB - how stable is phosphorylation once transferred on WB membrane? (reply: 6)
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western blot problems on getting signal - (reply: 5)
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Western Blotting - Old school - Looking for old protocol (reply: 3)
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Western Blots on media - (reply: 4)
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conditioned media for western - (reply: 1)
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Tween function in Western blot - (reply: 2)
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Western Blot Hela Cos7 - Show nearly identical genes of two related organisms same expression (reply: 4)
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Western blots - fuzzy below 100 kDa - (reply: 3)
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strange bands on western blot - (reply: 2)
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Western loading control for secreted protein? - (reply: 3)
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Help with western blotting - protein not running in to seperating gel (reply: 1)
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Nuclear extract - Protocol for nuclear extract for WB (reply: 2)
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MDR western - (reply: 1)
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Immunoblot (western) resolution vs electrophoresis resolution - (reply: 1)
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Western problem - very challenging - distorted band, non-reproducible, generally awful (reply: 5)
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Loading controls for Western blot - (reply: 2)
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help on western blots using plasma or serum - (reply: 1)
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Western transfer time: Is it crucial for big proteins? - anomaly HELP (reply: 2)
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Western blot background and specificity - How good is my antiserum? (reply: 1)
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Western Blot troubleshooting: band is too high - LiCor imaging of Alpha tubulin membranes (reply: 6)
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white bands on western blots - (reply: 1)
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help - small proteins and western blotting - (reply: 23)
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Cell lysis for westerns? - Immediately after harvest?? (reply: 8)
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Where did the protein go? - Low protein reading in WB lysates (reply: 4)
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Streaks appearing between lanes in my western blot - (reply: 3)
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Some lanes see bands,other no, strange! - western blotting questions (reply: 10)
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Western band on blot uncapturable by film - (reply: 3)
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Harvesting cells for Western - what protease inhibitors do you add to PBS? - (reply: 4)
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Western blots on contaminated cell cultures? - Mycoplasma (reply: 1)
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How to make western results clean??? - (reply: 3)
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siRNA control by Western blot - how do I get equal loading concentration? (reply: 7)
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Western blotting - (reply: 2)
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Western transfer problem - (reply: 3)
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orthovanadate in transfer buffer for WB? - immunodetection of phospho ERK1/2 (reply: 6)
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Western blot bands look like dumbells - (reply: 2)
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Protocol for histone acid extraction for Western blot - (reply: 13)
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western blotting and histone acetylation - (reply: 2)
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Western blot-quantitation - (reply: 4)
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BSA purity for Western Blot? - What BSA to use? (reply: 1)
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western blot forgot to transfer - (reply: 2)
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antibody problems in western - Got similiar western signals for three different p (reply: 8)
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WB: one single band but at the wrong size... - what to make of it? (reply: 4)
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uneven western blot - (reply: 1)
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<10kD protein in Western - (reply: 3)
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Western Blot question: cutting membrane into strips - (reply: 2)
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Too many bands on Western blot - (reply: 5)
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anti-goat:anti-western blotting??? - Western blotting! (reply: 3)
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protiens with similar MB but shift on the western blot - (reply: 1)
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Distorted bands on Western Blot - (reply: 10)
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western troubles - any help with connexins western (reply: 1)
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Western Blots: no signal - low or no detection signal (reply: 8)
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Protein isolation for western - (reply: 8)
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western blot samples trouble - (reply: 6)
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Is it ok to re-use Western Transfer Buffer - (reply: 4)
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Problems in Western blot ! - need help for my western blot (reply: 5)
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Sonicating cell lysate for Westerns - (reply: 2)
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Li-Cor WB optimisation - WB background at y680 of Li-Cor (reply: 1)
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Problem with TCA and western blot - (reply: 3)
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HELP: Western Blot problem - (reply: 3)
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western of a big protein - (reply: 2)
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Immunoblot (Western Blot) Question - Trying to nut out a question my demonstrator asked (reply: 1)
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Short Half-life of ECL on Western Blot? - Recurrent problem of short ECL half-life (reply: 2)
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Help on protein integrity Western Blot - Sample preparation and detection (reply: 6)
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can i freeze cell lysates for western blotting? - (reply: 3)
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question - anti Ha western blotting (reply: 1)
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lysis buffer for western blot - lysis buffer (reply: 3)
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western blot trouble shoot-plz help - (reply: 5)
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Western Blot - (reply: 1)
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enhancing western blot signals - incubation alternatives (reply: 3)
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p-Smad2 problems in western blot - TGF-beta and p-Smad 2 (reply: 1)
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Trouble with Western Blot Transfer (Poor transfer) - Could the protein isolation or homogenization be the cause? (reply: 4)
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apolipoprotein E identification via Westerns - (reply: 1)
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Far Western again... - (reply: 1)
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Far western - (reply: 3)
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Western blot detection other than antibody? - (reply: 5)
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Western blot problem - Western blot (reply: 5)
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loading control for western blots - (reply: 1)
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Putting more than one membrane in a container... - Western (reply: 2)
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How to determine band intensity of Western blot? - High School Student in need of help for the Science Fair! (reply: 8)
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Bands on Western are not sharply defined - (reply: 6)
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phastgels for Western blotting - (reply: 1)
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Western for membrane bound receptor - (reply: 1)
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Western blot - Pounceau S staining problem - (reply: 16)
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western blot - transfer trouble (reply: 5)
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total protein in Western blotting - (reply: 1)
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semiquantitative western blotting - (reply: 1)
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bone for western blotting - bone processing for western blotting (reply: 4)
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ECL Western blotting detection kit - (reply: 5)
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densitometry of westerns - (reply: 4)
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western blotting with anti-goat HRP - bands after ECL but only secondary antibody (reply: 1)
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Nonspecific Binding on Western Blot - HELP (reply: 10)
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Extraction of membrane proteins and western on them - (reply: 3)
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MAPK P38 - troubles with western blot (reply: 8)
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Western Blot - signal doesn't show up on film - (reply: 8)
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Expression of Proteins for western blot - (reply: 1)
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Western imaging analysis - (reply: 1)
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Western Blot after Coomassie stain of antibody? - Western Blotting after Coomassie stain? (reply: 3)
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too many bands in western blotting - (reply: 2)
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western blotting, (GFP is there - But where's the protein I want?) (reply: 3)
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western blotting using alkaline phosphatase - (reply: 4)
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western blotting - (reply: 1)
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IP/Western complication - Trying to pull down 2 conjoined proteins (reply: 2)
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Formalin Fixed Tissue westerns - (reply: 1)
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Western blot question - (reply: 1)
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High background in Western blot - (reply: 28)
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western blot problem - Blank film from WB with ECL (reply: 2)
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Double Bands in Western Blotting - Please Help (reply: 2)
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Western blot for 2D gel - (reply: 1)
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Western blotting quantification and normalisation - (reply: 3)
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TCR Zeta Chain & Phospho-species - immunoprecipitation and WB (reply: 1)
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aspecific band of BSA in western blot - western blotting (reply: 1)
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Has anybody done western blot on calmodulin? - (reply: 1)
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Western Blot - Cross reactivity of first Ab (reply: 1)
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Neg control fopr western? - (reply: 3)
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Western Blot for Elastin orother Matrix Proteins - (reply: 1)
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Western blotting - protein quantification and free-software (reply: 2)
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How the West(ern) was lost - (reply: 3)
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VEGF Western blots - (reply: 4)
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Western Blotting - (reply: 4)
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labeling on southern, northern and western blot - (reply: 1)
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about Western Blot - (reply: 3)
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Trouble detcting phosphJNK by western - (reply: 1)
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Electroblotting of proteins(western) - (reply: 2)
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Western - (reply: 2)
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