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Western Blotting - Old school - Looking for old protocol (Feb/01/2006 )

Hi everyone,

i am in need for a western blotting protocol which works without a special device... i'm talking about this old school technique with pressure only. i would be extremly happy if some one provided a protocol/link/something


is there nothing in Maniatis? not that I have looked, I am making an assumption


western blot has always been with current


I disagree...I will look for a reference on capillary transfer of Westerns but I am sure that it used to be done

all right, check this here

scroll down about 3/4 page