Fluorescent detection of western blots - (Feb/26/2008 )

Has anybody tried Alexa Fluor antibodies for western blot, your inputs will be appreciated.

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I am pretty sure it works. You need the right imaging system.

DIGE system is based on fluorescence labelling of proteins in a 2D gel system. You have 2 or more samples labeled with fluorescence dyes which can be used to detect changes among samples.

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Our lab has used AlexaFluor secondary antibodies (430 and 635) with the Storm860 imager.

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Thanks, I will be using the Syngene G: Box chemi 16.
Could you please mail me the blocking conditions was it 5% Non fat milk or 3% BSA and also the secondary Ab dilution. Thanks again.

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