Used western blot film-how to do stripping? - (May/19/2008 )
Hi guys,
what protocols do you use to strip off the 2nd antibody after the film has been incubated with ECL solution? I have never done this. Some protocols use b-meca and sds while some use glycine at a high temperature. Could you guys tell me how you do it in the lab? Thanks,
-Stressguy-
QUOTE (Stressguy @ May 19 2008, 01:55 PM)
Hi guys,
what protocols do you use to strip off the 2nd antibody after the film has been incubated with ECL solution? I have never done this. Some protocols use b-meca and sds while some use glycine at a high temperature. Could you guys tell me how you do it in the lab? Thanks,
what protocols do you use to strip off the 2nd antibody after the film has been incubated with ECL solution? I have never done this. Some protocols use b-meca and sds while some use glycine at a high temperature. Could you guys tell me how you do it in the lab? Thanks,
http://search.vadlo.com/b/q?keys=stripping...mp;sn=158621799
hth/
-cellcounter-