Protocol Online logo
Top : Forum Archives: : Immunology and Histology

lysate for ELISA can be used for WESTERN? - (Jun/13/2006 )


Hi, I am a new one for ELISA.

Currently I am considering making protein from liver for ELISA and Western-blot. As the amount of my sample is very limited, if I prepare the lysate for ELISA, can I also use those lysate for Western?

Thanks for your information!

smile.gif smile.gif


I guess it should work.


hi BLUE-SNOW ! that depends on the type of detergent you are using for the lysis. but most protocols suggest a non ionic detergents and this works well for both ELISA as well as western blotting