WB vs ELISA ? - (Nov/18/2007 )

does any antibody that is provided for WB works for ELISA?

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As long as the (linear) epitope is on the surface, you should get some response wit hit. However, the epitope could be in such a position that it's not always accessible to the Ab, or it could be internal, and therefore complpetely hidden. If your question comes for a lack of funds, all you can do is give it a shot and see what happens.

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in many cases companies test their Ab´s for suitability of immunologic techniques; Ab´s for Wb detect epitopes in denaturized polypeptides, ELISA detects epitopes of native polypeptides/proteins; denaturized proteins offer more or different epitopes than native ones

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