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western blotting of tissues - (Feb/21/2006 )

I have a tumor, how do i get from tumor to protein?Step by step please help ? Furthermore what does homegoinize mean?If anyone has a detailed protocol?



Try going to the "protocol" section on this site (link at top left of page), that is what it is for! There are plenty of methods under molecular biology, just follow one for going from cells to protein. Homogenize means to make into a homogenous mixture i.e. a mixture thast is all the same (no lumps of stuff, well mixed etc).

Failing that look in a text book such as "Molecular cloning: a laboratory manual".