Blokcing step of Western blotting - (Feb/21/2007 )

Hi everyone,

Can anyone tell me is there any difference in using TBS or TBS w/Tween 20 during the blocking step of western blotting?
Which one is recommended to mix up with non-fat milk?

-HenryTcby-

QUOTE (HenryTcby @ Feb 21 2007, 03:04 PM)

Hi everyone,

Can anyone tell me is there any difference in using TBS or TBS w/Tween 20 during the blocking step of western blotting?
Which one is recommended to mix up with non-fat milk?

Tween 20 serves as a non-protein blocking agent, you can mix it with non-fat dry milk or leave it; washing steps after blocking are recommended to perform with TTBS

-The Bearer-

we use TBS with tween for preparing our blocking solution.

-scolix-

we do too.

-Madrius-

we block without tween. we use tween in our antibody and wash solutions. it helps reduce non-specific binding.

-mdfenko-

It's useless to put tween in blocking, however it won't be bad. So, do as it is more convenient for you.

-Missele-