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how larg should my tissue sample be for western blotting? - (Jan/06/2007 )

i am starting a research on oral cancer. and my samples are going to be analysed with western blot technique.
my question is how larg should my sample be for this job!

-hamed s-

20-40ug should be sufficient. I normally use 20. But it may depend upon your protein and the quality of your antibody.