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about Western Blot - (Aug/06/2002 )

1.why amido black can bind total transfer membrane protein?
2.ddH2O or ethanol,which one can add to the lower gel to prevent gas formation?
can you tell me,thanks

-yangwenjyh-

on gas formation just make your gel under a vaccuum then no bubbles

-bigbambu-

how to keep my gel in a vaccum condition?
i have water and alcohol only.
do i need any equipment?
thanks

-yangwenjyh-

are you making sds page gels ? triphasic? if so degas in a beaker with a vaccuum source attached then make sure the glass is TOTALLY clean and pour down one side of rig with pastuer pipette very slowly and carefully. it takes a little more time but it works for me.

-bigbambu-