Supernatant for western - (Jul/11/2008 )
Hello
I'm attempting to collect supernatant from fibroblast cell culture for western blotting, so far unsuccessfully.
Does anybody have a protocol I can try?
I'm using 24-well cell culture plates and I would like to verify details as number of cell/well, minimal amount of medium, and collecting conditions.
Thanks,
Liane
-liane-
What protein(s) are you looking at?
-Minnie Mouse-
Dilution will be your enemy in this, you need some way of concentrating the proteins present in the medium before you will be able to see them on a western.
-bob1-
My control is albumin
I'm looking for IL-1 and TGF
-liane-