Neutralizing Antibodies - on westerns and IF (May/23/2008 )
Anybody uses neutralizing antibodies on western blots?
Can you tell me what are they and how do they work??
I would also like to know this.
Generally, neutralizing antibodies are used in cell culture assays where you want to block receptor-ligand binding. It just occupies a receptor or ligand's binding surface and does not allow it to participate in binding with right partner, thereby blocking signal transduction.
For Westerns, if you want to confirm a specific band when you have many non-specifics, you block the protein-antibody binding by using the peptide against which the antibody was raised. Same is true for IP.