Western blotting with blood serum samples - (Sep/16/2008 )

Dear all,

A colleague has recently tried to run a Western blot using blood serum samples, and upon adding sample buffer amd boiling, the samples beacme so viscous (???coagulated) that it was not possible to run the sds-page.... any ideas????

Many thanks

James

DNA?

the proteins aggregated during boiling.

heat at 65C for 10-20 minutes instead of boiling.