Western blotting with blood serum samples - (Sep/16/2008 )
A colleague has recently tried to run a Western blot using blood serum samples, and upon adding sample buffer amd boiling, the samples beacme so viscous (???coagulated) that it was not possible to run the sds-page.... any ideas????
the proteins aggregated during boiling.
heat at 65C for 10-20 minutes instead of boiling.