bone for western blotting - bone processing for western blotting (Jan/03/2005 )
Hello there
Is there anyone who detected some proteins in bone by employing the western blotting. If yes, how can I process the bone to make it suitable for western blotting. Answers are really appreciated. Give me an answer and save my life, mates.. thanks. Engin from Turkey.
-eulukaya-
Are you interested in the marrow or another part of bone? If it's the marrow I can help - otherwise I have no idea how to process calcified bone for westerns.
-MaximinaNYC-
QUOTE (eulukaya @ Jan 3 2005, 02:19 PM)
Hello there
Is there anyone who detected some proteins in bone by employing the western blotting. If yes, how can I process the bone to make it suitable for western blotting. Answers are really appreciated. Give me an answer and save my life, mates.. thanks. Engin from Turkey.
Is there anyone who detected some proteins in bone by employing the western blotting. If yes, how can I process the bone to make it suitable for western blotting. Answers are really appreciated. Give me an answer and save my life, mates.. thanks. Engin from Turkey.
It's probably too late now, but, anyway - you need a hard tissue homogeniser to prepare the proteins. Any standard protein isolation buffer will do fine.
-Pallas-
Interesting Pallas, good to know ... Could you grind the bone with a mortar and pestle or would you need the homogenizer itself?
-MaximinaNYC-
QUOTE (MaximinaNYC @ Apr 19 2005, 04:31 PM)
Interesting Pallas, good to know ... Could you grind the bone with a mortar and pestle or would you need the homogenizer itself?
I've never tried mortar & pestle, sorry, but I'd say - no.
-Pallas-