loading control for western blots - (Feb/14/2005 )
could anyone tell me what the best loading control is for western blotting. I work on yeast and so I'm wondering if people use actin or is there something very specific to yeast people.
just thought that I would answer my own question
Actin is not cell cycle regulated and therefore a good loading control for cell cycle work. others including phospho-glycerate kinase and GAPDH do show some differences during the cell cycle