Western Blot of PKCalpha - (Apr/13/2006 )

I am currently performing western blotting of PKCalpha. I am getting a double band at what is the expected molecular mass. I've noticed in one paper that when you have subpopulations of activated and inactivated PKCalpha you get a double band but I have no evidence that this is the case in my experiment.

I have performed no treatment on my cells. Would I expect to see both populations?

Anyone done this before and seen something similar.

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yes, you should expect to see both populations because they both exist inside the cell as long as it continues to function.

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