How to quantify Western blot results/bands? - with ImageJ? Scion? Other programs? (Nov/12/2007 )
Hello everyone,
This time I need all of your expertise in quantifying western blots.
I know there were already some posts in the past concerning this,, but the spoiled brat I am 
, I need more comprehensive manual/instruction on what exactly what to do, starting from after I finish developing the blots with 2ndary antibody substrates.
thanks a bunch!!!!
-chrismartin-
never mind!!! i've found some very helpful infos, e.g.:
http://www.lukemiller.org/journal/2007/08/...ts-without.html
just in case some of you are in need as well.
viva western blot!
-chrismartin-
use ImageJ, for free
http://www.protocol-online.org/forums/inde...?showtopic=8201
-tryptofan-
QUOTE (chrismartin @ Nov 12 2007, 04:05 AM)
Hello everyone,
This time I need all of your expertise in quantifying western blots.
I know there were already some posts in the past concerning this,, but the spoiled brat I am , I need more comprehensive manual/instruction on what exactly what to do, starting from after I finish developing the blots with 2ndary antibody substrates.
thanks a bunch!!!!
This time I need all of your expertise in quantifying western blots.
I know there were already some posts in the past concerning this,, but the spoiled brat I am
, I need more comprehensive manual/instruction on what exactly what to do, starting from after I finish developing the blots with 2ndary antibody substrates.thanks a bunch!!!!
SI UNIT for length.........metre, centimetre, millimetre......
SI UNIT for weight........Kilogramme, gramme, milligramme...
INTERNATIONALLY RECOGNISED SI UNIT FOR QUANTIFYING DENSITOMETRY PLEASE...........
-Rhombus-
QUOTE (Rhombus @ Nov 13 2007, 03:20 PM)
QUOTE (chrismartin @ Nov 12 2007, 04:05 AM)
Hello everyone,
This time I need all of your expertise in quantifying western blots.
I know there were already some posts in the past concerning this,, but the spoiled brat I am , I need more comprehensive manual/instruction on what exactly what to do, starting from after I finish developing the blots with 2ndary antibody substrates.
thanks a bunch!!!!
This time I need all of your expertise in quantifying western blots.
I know there were already some posts in the past concerning this,, but the spoiled brat I am
, I need more comprehensive manual/instruction on what exactly what to do, starting from after I finish developing the blots with 2ndary antibody substrates.thanks a bunch!!!!
SI UNIT for length.........metre, centimetre, millimetre......
SI UNIT for weight........Kilogramme, gramme, milligramme...
INTERNATIONALLY RECOGNISED SI UNIT FOR QUANTIFYING DENSITOMETRY PLEASE...........
densitometry has no units; it is a numeric interpretation of gray scale ranging from 0-255 multiplied with number of pixels; if you like you can define "arbitrary units"
-The Bearer-